48/192-Test Fluorescent RNase Contamination Assay Kit
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48/192-Test Fluorescent RNase Contamination Assay Kit

Cat.No: NATR-HMM-0215 Datasheet

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Product Details Related Products
Product Name 48/192-Test Fluorescent RNase Contamination Assay Kit
Catalog No. NATR-HMM-0215
Description Alta DiagnoTech fluorescent RNase assay kit is designed to evaluate RNase contamination in laboratory samples.
Intended Use For detecting RNase contamination in samples.
Detection Method Fluorescence measurement using VIC or HEX detection
Reaction Conditions / Protocol When using a compatible qPCR instrument, run 30 cycles at 37°C for 1 minute with fluorescence acquisition in the VIC or HEX channel using a 100 µL reaction. Use raw fluorescence data with cycle 1 as RFU0 and cycle 30 as RFU30.
Components / Formulation RNA probe solution and RNase standard.
Shelf Life 12 months unopened; 6 months after opening.
Package Specifications Available in 48- and 192-test formats.
Product Form Fluorescent nuclease assay kit
Activity / Unit Definition One RNase A unit produces an absorbance increase of 1.0 at 260 nm with yeast RNA at pH 5.0 and 37°C; 50 RNase A units equal one Kunitz unit.
Compatibility Compatible with fluorescence microplate readers and qPCR instruments equipped with a VIC or HEX channel.
Application Notes / Precautions Aliquot the probe solution according to single-use volume, protect from light and avoid repeated freeze-thaw cycles. Add samples promptly, mix all reagents thoroughly, dispense to the bottom of wells and avoid splashing or bubbles. Instrument gain should be optimized before first use.
Alias RNase assay kit (fluorescence)
Product Type RNase contamination detection reagent
Function / Efficacy Detects RNase activity as an indicator of sample contamination.

For research use only, not for clinical use.

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