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| Product Name | 48/192-Test Fluorescent DNase Contamination Assay Kit |
| Catalog No. | NATR-HMM-0210 |
| Description | Alta DiagnoTech fluorescent DNase assay kit is designed to evaluate DNase contamination in laboratory samples. |
| Intended Use | For detecting DNase contamination in samples. |
| Detection Method | Fluorescence measurement using FAM detection |
| Reaction Conditions / Protocol | When using a compatible qPCR instrument, run 30 cycles at 37°C for 1 minute with fluorescence acquisition in the FAM channel using a 100 µL reaction. Use raw fluorescence data with cycle 1 as RFU0 and cycle 30 as RFU30. |
| Components / Formulation | DNA probe solution and DNase standard. |
| Shelf Life | 12 months unopened; 6 months after opening. |
| Package Specifications | Available in 48- and 192-test formats. |
| Product Form | Fluorescent nuclease assay kit |
| Activity / Unit Definition | One DNase I unit completely degrades 1 µg pBR322 DNA in 10 minutes at 37°C in DNase I reaction buffer; one unit equals 0.3 Kunitz unit. |
| Compatibility | Compatible with fluorescence microplate readers and qPCR instruments equipped with a FAM channel. |
| Application Notes / Precautions | Aliquot the probe solution according to single-use volume, protect from light and avoid repeated freeze-thaw cycles. Add samples promptly, mix all reagents thoroughly, dispense to the bottom of wells and avoid splashing or bubbles. Instrument gain should be optimized before first use. |
| Alias | DNase assay kit (fluorescence) |
| Product Type | DNase contamination detection reagent |
| Function / Efficacy | Detects DNase activity as an indicator of sample contamination. |
For research use only, not for clinical use.
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