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| Product Name | 300 nm Streptavidin Magnetic Beads at 10 mg/mL for Biotin Capture |
| Catalog No. | IRMGM-0058 |
| Description | Alta DiagnoTech 300 nm superparamagnetic beads carry covalently immobilized streptavidin for rapid capture and magnetic isolation of biotinylated ligands. |
| Intended Use | Capture of biotinylated antibodies, nucleic acids, proteins and other ligands in manual or automated workflows. |
| Principle / Technology | High-affinity streptavidin-biotin binding with an association constant of approximately 10^-15. |
| Detection Method | Magnetic capture and separation |
| Sample Type | Biotinylated antibodies, oligonucleotides, DNA probes and proteins |
| Performance Range / Specifications | The 300 nm format binds approximately 450 pmol/mg of a 24-nt biotinylated oligonucleotide and 15 µg/mg of biotinylated IgG. The same series comparison lists 1, 2 and 5 µm formats at 500, 400 and 400 pmol/mg oligonucleotide; 20, 20 and 15 µg/mg IgG; and 1,100, 1,000 and 800 pmol/mg free biotin, respectively. |
| Sensitivity / LOD | Application examples detect biotinylated IgG at 6.25 ng/mL and biotinylated DNA probe at 6.25 fmol/mL. |
| Storage Conditions | 2-8 °C |
| Shelf Life | 2 years |
| Package Specifications | 1, 10 or 100 mL |
| Product Form | Magnetic bead suspension |
| Key Features | High biotin-binding activity, rapid magnetic response, uniform morphology, broad experimental compatibility and automated high-throughput capability. |
| Concentration | 10 mg/mL |
| Product Type | Streptavidin magnetic beads |
| Function / Efficacy | Bind and isolate biotinylated biomolecules with low background adsorption. |
| Storage Buffer | 1× PBS containing 0.1% w/v BSA and 0.1% v/v ProClin 300 |
| Analytes / Targets | Biotinylated antibodies, nucleic acids and proteins |
| Matrix | Superparamagnetic polymer microspheres |
| Particle Size | 300 nm |
| Ligand / Bonded Phase | Streptavidin |
| Ligand Coupling Method | Covalent immobilization on the bead surface |
For research use only, not for clinical use.
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