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| Product Name | 30-Second-Activation Hot-Start Probe qPCR Master Mix for Multiplex DNA |
| Catalog No. | NATR-HMM-0248 |
| Description | Alta DiagnoTech probe qPCR master mix uses antibody-modified Taq polymerase and an optimized buffer for sensitive multiplex DNA amplification. |
| Intended Use | For multiplex probe-based quantitative PCR using DNA templates. |
| Principle / Technology | Antibody blockade suppresses polymerase and double-strand-specific 5′→3′ exonuclease activities during setup. A 30-second activation at 95°C releases enzyme activity, while UDG chemistry reduces carryover contamination. |
| Detection Method | Probe-based real-time fluorescence detection |
| Reaction Conditions / Protocol | Activate at 95°C for 30 seconds before amplification. |
| Components / Formulation | Optimized reaction buffer, antibody-modified hot-start Taq DNA polymerase and UDG carryover-control chemistry. |
| Storage Conditions | Store at -25°C to -15°C. |
| Package Specifications | Available in 100-, 500-, 1,000- and 5,000-reaction formats. |
| Product Form | Liquid probe qPCR master mix |
| Shipping Conditions | Ship at 0°C or below. |
| Compatibility | Accommodates varied template types, template GC content and primer melting temperatures. |
| Product Type | Antibody-modified probe qPCR master mix |
| Function / Efficacy | Reduces nonspecific amplification and primer-dimer formation while improving qPCR sensitivity and specificity. |
For research use only, not for clinical use.
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