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| Product Name | 293 Plus Transfection Reagent, HEK293 Series Optimised |
| Catalog No. | RERE-0322 |
| Description | A next-generation cationic liposome reagent developed mainly for the adherent 293 family, including HEK293, HEK293T, HEK293A and 293FT cells. It mediates highly efficient transient delivery of plasmids, RNA and oligonucleotides into 293 series cultures, typically reaching about 90% efficiency with low cytotoxicity, good reproducibility, simple handling and modest cost. Being chemically defined, the formulation contains no animal-derived components. Serum and antibiotics present during transfection do not compromise performance, sparing cells the damage that often accompanies serum removal with other cationic lipid systems. Protein expression generally plateaus 24 to 48 hours after delivery. While the reagent can be applied to other adherent lines, this is only advisable for easily transfected cells in high-sensitivity assays such as luciferase reporting. |
| Intended Use | Transient delivery of plasmid DNA, RNA and oligonucleotides into adherent HEK293, HEK293T, HEK293A and 293FT cultures; adaptable to other adherent lines in high-sensitivity reporter assays. |
| Principle / Technology | Cationic liposomes condense nucleic acid cargo into complexes that adherent 293 series cells take up readily, producing high transient expression without serum withdrawal. |
| Specificity | Sterile; BioReagent grade; molecular biology grade. One millilitre supports approximately 33 x 10 cm dishes, 100 x 6 cm dishes, 200 wells of a 6-well plate, 500 wells of a 12-well plate, 1000 wells of a 24-well plate, 2000 wells of a 48-well plate or 5000 wells of a 96-well plate. |
| Reaction Conditions / Protocol | Seed cells the day before so they reach 70-80% confluence at delivery. Dilute DNA and reagent separately in serum-free medium, combine in a DNA-to-reagent ratio of roughly 1:2 by mass to volume, incubate 10-20 minutes at room temperature, then add the mixture dropwise to the culture. Assays can be read 24-48 hours later, or stable selection started with appropriate antibiotics. |
| Storage Conditions | 2-8°C; 12 months. |
| Package Specifications | 100 µL, 500 µL, 1.5 mL |
| Key Features | Approximately 90% efficiency on 293 series cells; animal origin free; serum and antibiotic tolerant; low cytotoxicity; EGFP-based performance checks possible within 48 hours. |
For research use only, not for clinical use.
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