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| Product Name | 293 Cell Suspension Transfection Reagent, High-Density Culture |
| Catalog No. | ATR-CTR-0024 |
| Description | High-performance transfection reagent for HEK293 suspension cells at high density (3-6×10⁶ cells/mL). Enables large-scale transient protein production in FreeStyle 293-F and Expi293F cells with IgG titers up to 1 g/L. Validated for AAV and lentivirus manufacturing at 10 L scale. Compatible with commercial serum-free 293 media. |
| Intended Use | Large-scale transient HEK293 transfection; recombinant protein/antibody production; AAV and lentivirus manufacturing; adenovirus production; membrane protein expression. |
| Principle / Technology | Cationic lipid delivery optimized for suspension HEK293 at high density; lipid composition facilitates complex formation in high-ionic-strength media and rapid uptake |
| Detection Method | For 1 L at 3×10⁶ cells/mL: prepare 1 mg DNA + 2 mL reagent in transfection buffer; complex 10-15 min; add to cells; harvest day 4-7 |
| Sample Type | FreeStyle 293-F, Expi293F, HEK293-6E, suspension-adapted HEK293T |
| Reaction Conditions / Protocol | Complex: 10-15 min RT; no medium change; expression: 4-7 days; optional feed at day 2-3 |
| Components / Formulation | Cationic lipid blend in buffer, transfection buffer (low-EDTA), scale-up protocol |
| Storage Conditions | 2-8 °C; do not freeze; warm to RT and vortex before use |
| Shelf Life | 12 months; 6 months after opening |
| Package Specifications | 1 mL, 5 mL, 20 mL, 100 mL |
| Product Form | Clear colorless to pale yellow sterile liquid |
| Key Features | Optimized for 3-6×10⁶ cells/mL; >90% efficiency; IgG titers up to 1 g/L; 1:2 DNA-to-reagent ratio; validated 10 L AAV production; compatible with hydrolysate feeds |
| Purity | Lipid ≥98%; endotoxin <0.05 EU/mL; particle size 200-400 nm post-complexation |
| Concentration | As specified (typically 1 mg/mL for lipid reagents) |
| Activity / Unit Definition | Transfection efficiency: ≥70% in HEK293 cells with control plasmid at recommended ratio |
| Molecular Weight | Varies by formulation as specified in product documentation |
| Source / Origin | Synthetic cationic lipids and polymers; recombinant viral components where applicable |
| pH Range / Optimal pH | Lipoplex formation optimal at pH 7.0–7.4 |
| Shipping Conditions | Cold pack 2-8 °C; do not freeze; protect from light |
| Expiration Date / Stability | 12 months at 2-8 °C; 6 months after opening |
| Regulatory / Compliance | For laboratory and research use only; RUO; ACF; ISO 9001 |
| Compatibility | Compatible with serum-containing and serum-free culture media; check product-specific compatibility |
| Recommended Buffer System | Serum-free medium or PBS for complex formation; complete medium for post-transfection culture |
| Application Notes / Precautions | Optimize reagent-to-DNA ratio per cell type; avoid antibiotic during transfection; monitor cytotoxicity |
| Batch-to-Batch Consistency | Transfection efficiency within ±15% of reference lot on HEK293 standard |
For research use only, not for clinical use.
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