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| Product Name | 2× One-Step RT-qPCR SYBR Master Mix with Pre-Added ROX |
| Catalog No. | NATR-HMM-0238 |
| Description | Alta DiagnoTech 2× RT-qPCR SYBR master mix is a one-tube intercalating-dye reagent for gene-specific RNA quantification. |
| Intended Use | For one-step real-time quantitative PCR using gene-specific primers and SYBR Green I fluorescence. |
| Principle / Technology | Reverse transcription and qPCR occur in one closed tube using a thermostable reverse transcriptase, hot-start Taq polymerase, SYBR Green I dye, nonspecific-amplification suppressors and specificity-promoting components. |
| Components / Formulation | A 2× master mix with SYBR Green I chemistry and pre-added ROX reference dye. |
| Storage Conditions | Store protected from light at -30°C to -15°C. |
| Package Specifications | Available in 100- and 500-reaction formats. |
| Shipping Conditions | Ship at 0°C or below. |
| Compatibility | Pre-added ROX supports most real-time PCR instruments without separate ROX adjustment. |
| Application Notes / Precautions | Use RNase-free consumables, a dedicated pipette and filter tips. Avoid bubbles; centrifuge again if bubbles form. Fully thaw and mix before use, do not combine different reagent lots, ensure tube caps are secure and prepare reactions in a clean bench because of the high assay sensitivity. |
| Product Type | One-step RT-qPCR SYBR master mix |
| Function / Efficacy | Provides sensitive and specific one-tube RNA quantification while reducing handling and contamination risk. |
For research use only, not for clinical use.
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