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| Product Name | 2-8°C Lyophilized LAMP Probe Beads for 30-Cycle DNA Amplification |
| Catalog No. | NATR-HMM-0225 |
| Description | Alta DiagnoTech LAMP probe master mix is supplied as lyophilized beads containing reaction buffer, Bst DNA polymerase enzyme mix and lyoprotective components. |
| Intended Use | For probe-based DNA isothermal amplification. |
| Principle / Technology | Each lyophilized bead supplies the core buffer and enzyme system for probe LAMP; users add primer/probe mix and DNA template before amplification. |
| Reaction Conditions / Protocol | Use one bead with 5 µL 10× primer/probe mix and 45 µL DNA template. The 10× mix contains 16 µM FIP/BIP, 2 µM F3/B3, 4 µM Loop F/B and 4 µM probe. Run 30 cycles at 65°C for 60 seconds with FAM fluorescence collection; optimize from 62°C to 68°C when required. |
| Components / Formulation | Reaction buffer containing Mg2+ and dNTPs, Bst DNA polymerase enzyme mix and lyophilization protectants. |
| Storage Conditions | Store at 2-8°C. |
| Shelf Life | 12 months |
| Package Specifications | Available in 96-, 960- and 9,600-test formats, configured as 8 beads per strip with 12, 120 or 1,200 strips. |
| Product Form | Lyophilized beads |
| Shipping Conditions | Ship at 2-8°C. |
| Compatibility | Use DNA dissolved in DEPC-treated water and optimize reaction temperature for the primer set. |
| Application Notes / Precautions | Use standardized workflow for reaction setup, sample handling and loading. Prepare the reaction mix in a clean bench and add template in a separate room or ventilated enclosure to reduce false-positive contamination. |
| Product Type | Lyophilized LAMP probe master mix |
| Function / Efficacy | Provides a refrigerated, ready-to-reconstitute probe LAMP system for DNA amplification. |
For research use only, not for clinical use.
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