2-8°C Lyophilized LAMP Probe Beads for 30-Cycle DNA Amplification
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2-8°C Lyophilized LAMP Probe Beads for 30-Cycle DNA Amplification

Cat.No: NATR-HMM-0225 Datasheet

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Product Name 2-8°C Lyophilized LAMP Probe Beads for 30-Cycle DNA Amplification
Catalog No. NATR-HMM-0225
Description Alta DiagnoTech LAMP probe master mix is supplied as lyophilized beads containing reaction buffer, Bst DNA polymerase enzyme mix and lyoprotective components.
Intended Use For probe-based DNA isothermal amplification.
Principle / Technology Each lyophilized bead supplies the core buffer and enzyme system for probe LAMP; users add primer/probe mix and DNA template before amplification.
Reaction Conditions / Protocol Use one bead with 5 µL 10× primer/probe mix and 45 µL DNA template. The 10× mix contains 16 µM FIP/BIP, 2 µM F3/B3, 4 µM Loop F/B and 4 µM probe. Run 30 cycles at 65°C for 60 seconds with FAM fluorescence collection; optimize from 62°C to 68°C when required.
Components / Formulation Reaction buffer containing Mg2+ and dNTPs, Bst DNA polymerase enzyme mix and lyophilization protectants.
Storage Conditions Store at 2-8°C.
Shelf Life 12 months
Package Specifications Available in 96-, 960- and 9,600-test formats, configured as 8 beads per strip with 12, 120 or 1,200 strips.
Product Form Lyophilized beads
Shipping Conditions Ship at 2-8°C.
Compatibility Use DNA dissolved in DEPC-treated water and optimize reaction temperature for the primer set.
Application Notes / Precautions Use standardized workflow for reaction setup, sample handling and loading. Prepare the reaction mix in a clean bench and add template in a separate room or ventilated enclosure to reduce false-positive contamination.
Product Type Lyophilized LAMP probe master mix
Function / Efficacy Provides a refrigerated, ready-to-reconstitute probe LAMP system for DNA amplification.

For research use only, not for clinical use.

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