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| Product Name | 15-Minute Mouse CD8+ T Cell Negative Isolation Kit for 5 x 10^8-10^9 Cells |
| Catalog No. | IRMGM-0074 |
| Description | Alta DiagnoTech mouse CD8+ T-cell kit depletes non-target cells from mouse spleen or other tissue-derived single-cell suspensions. |
| Intended Use | Isolation of mouse CD8+ T cells from spleen cells or single-cell suspensions prepared from other tissues. |
| Principle / Technology | Biotinylated monoclonal antibodies label non-CD8+ T cells, which are removed with streptavidin-coated magnetic beads. |
| Detection Method | Column-free magnetic negative selection |
| Sample Type | Mouse spleen cells and other tissue-derived single-cell suspensions |
| Performance Range / Specifications | Target-cell purity can exceed 95%, and post-isolation cell viability can exceed 99%. |
| Reaction Conditions / Protocol | Target cells can be isolated in approximately 15 minutes using a magnetic separator. |
| Components / Formulation | Biotinylated monoclonal antibody mixture and streptavidin-coated magnetic beads |
| Package Specifications | Formats for 5 x 10^8 or 1 x 10^9 cells |
| Product Form | Magnetic negative-selection cell isolation kit |
| Key Features | Fast 15-minute workflow, no separation column, high purity, high viability and retention of cell function. |
| Compatibility | Magnetic cell separator |
| Application Notes / Precautions | In a C57BL/6 spleen-cell example, CD8+ T-cell purity increased from 13.8% to 97.1% by flow cytometry using FITC anti-mouse CD8 clone 53-6.7. |
| Product Type | Mouse CD8+ T-cell negative-selection kit |
| Function / Efficacy | Deplete non-target cells to recover unlabeled mouse CD8+ T cells. |
| Analytes / Targets | Mouse CD8+ T cells |
For research use only, not for clinical use.
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