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| Product Name | 15-Minute Human CD3+ T Cell Negative Isolation Kit for Up to 10^9 PBMCs |
| Catalog No. | IRMGM-0080 |
| Description | Alta DiagnoTech human CD3+ T-cell kit removes non-target cells from peripheral blood mononuclear cells by magnetic negative selection. |
| Intended Use | Isolation of human CD3+ T cells from peripheral blood mononuclear cells. |
| Principle / Technology | Biotinylated monoclonal antibodies label non-CD3+ T cells, which are depleted with streptavidin-coated magnetic beads. |
| Detection Method | Column-free magnetic negative selection |
| Sample Type | Human peripheral blood mononuclear cells |
| Performance Range / Specifications | Target-cell purity can exceed 95%; recovered cells are stated to remain viable, functional and free of antibody or bead labels. |
| Reaction Conditions / Protocol | Target cells can be isolated in as little as 15 minutes using a magnetic separator. |
| Components / Formulation | Biotinylated monoclonal antibody mixture and streptavidin-coated magnetic beads |
| Package Specifications | Formats for 1 x 10^8, 5 x 10^8 or 1 x 10^9 cells |
| Product Form | Magnetic negative-selection cell isolation kit |
| Key Features | Fast workflow, no separation column, purity above 95% and recovery of unlabeled functional cells. |
| Compatibility | Magnetic cell separator |
| Application Notes / Precautions | In a human PBMC example, CD3+ T-cell purity increased from 69.1% to 97.9% by flow cytometry using PE anti-human CD3 clone OKT3. |
| Product Type | Human CD3+ T-cell negative-selection kit |
| Function / Efficacy | Deplete non-target cells to recover unlabeled human CD3+ T cells. |
| Analytes / Targets | Human CD3+ T cells |
For research use only, not for clinical use.
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