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| Product Name | 15-Minute All-in-One RT SuperMix for qPCR with gDNA Removal |
| Catalog No. | NATR-HMM-0175 |
| Description | An all-in-one qPCR reverse-transcription premix containing reverse transcriptase, thermolabile DNase and an optimized buffer for simultaneous genomic DNA removal and cDNA synthesis. |
| Intended Use | One-step genomic DNA removal and first-strand cDNA synthesis for qPCR. |
| Principle / Technology | Thermolabile DNase removes genomic DNA in the same tube in which reverse transcriptase converts RNA to stable cDNA. |
| Sample Type | Total RNA from animal cells, animal tissues and plant tissues. |
| Performance Range / Specifications | 100 reactions at 20 µL per reaction; genomic DNA removal and reverse transcription completed in approximately 15 minutes. |
| Specificity | Designed to reduce contamination risk and RNA degradation by eliminating transfer between separate treatment and RT steps. |
| Reaction Conditions / Protocol | Add RNA template to the all-in-one premix and complete the combined genomic DNA removal and reverse-transcription reaction in approximately 15 minutes. |
| Components / Formulation | All-in-one premix containing reverse transcriptase, thermolabile DNase and an optimized reaction buffer. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 100 reactions (20 µL per reaction). |
| Product Form | All-in-one RT supermix. |
| Quality Control | Evaluated by reverse-transcription efficiency and cDNA stability testing followed by qPCR. |
| Key Features | One-tube workflow; 15-minute operation; efficient reverse transcription; reduced handling; highly stable cDNA. |
| Shipping Conditions | Ship at or below 0°C. |
| Expiration Date / Stability | Generated cDNA remained stable after storage at 37°C for 7 days, at 4°C for 4 weeks, or after 30 freeze–thaw cycles, with qPCR ΔCt within 0.5 in the reported evaluation. |
| Compatibility | Resulting cDNA is compatible with dye-based and probe-based qPCR. |
| Application Notes / Precautions | The premix is optimized for qPCR-oriented cDNA synthesis. Follow the recommended template input and thermal program. |
For research use only, not for clinical use.
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