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100 bp DNA Ladder

Cat.No: NATR-HMM-0159 Datasheet

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Product Name 100 bp DNA Ladder
Catalog No. NATR-HMM-0159
Description A ready-to-load 100 bp-format DNA ladder prepared from defined double-stranded DNA fragments for routine agarose gel size estimation.
Intended Use DNA molecular size estimation during agarose gel electrophoresis.
Principle / Technology Defined double-stranded DNA fragments provide reference migration positions for estimating DNA fragment size.
Detection Method Agarose gel electrophoresis followed by nucleic acid staining and visualization.
Sample Type DNA electrophoresis samples.
Performance Range / Specifications The 500 bp fragment is supplied as an enhanced reference band.
Components / Formulation Purified plasmid-derived double-stranded DNA fragments premixed with blue loading buffer.
Storage Conditions Store at −20°C. After thawing, keep at 4°C and avoid repeated freeze-thaw cycles.
Package Specifications 50 reactions; 100 reactions.
Product Form Ready-to-load DNA molecular weight marker.
Key Features Clear and compact bands; accurate relative band mass; direct-loading format; stable at room temperature for up to 6 months.
Concentration 500 bp band: 100 ng per 5 μL; all other bands: 50 ng per 5 μL.
Expiration Date / Stability Reported to remain stable at room temperature for 6 months.
Compatibility Designed for agarose gel electrophoresis; not recommended for polyacrylamide gel electrophoresis.
Recommended Buffer System 1× TAE buffer with 0.8–2.0% agarose gel.
Application Notes / Precautions A voltage gradient of 4–10 V/cm is recommended. Use 5 μL per lane for the stated band masses, and avoid nuclease contamination and repeated freeze-thaw cycles.

For research use only, not for clinical use.

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