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| Product Name | 1 kb DNA Molecular Weight Marker |
| Catalog No. | NATR-HMM-0122 |
| Description | A ready-to-use DNA ladder consisting of 14 linear double-stranded DNA fragments ranging from 500 bp to 15,000 bp. The marker is designed for accurate molecular weight determination of larger DNA fragments separated by agarose gel electrophoresis. |
| Intended Use | Size estimation of double-stranded DNA fragments from 500 bp to 15 kb in agarose gel electrophoresis for genomic DNA analysis, cloning of large inserts, restriction mapping, and Southern blotting size determination. |
| Principle / Technology | DNA fragments of precisely defined lengths migrate through agarose gel at rates determined by their molecular weight. The regular spacing of fragments provides reference points for accurate interpolation of unknown fragment sizes across the entire range. |
| Detection Method | Agarose gel electrophoresis (0.5–1.5% gels) with ethidium bromide, SYBR Safe, SYBR Green, GelRed, or other nucleic acid stains; UV or blue-light transillumination |
| Sample Type | Double-stranded DNA samples including plasmid DNA, genomic DNA digests, long-range PCR products, and purified large fragments |
| Performance Range / Specifications | Fragment sizes: 500, 1,000, 1,500, 2,000, 2,500, 3,000, 3,500, 4,000, 5,000, 6,000, 7,000, 8,000, 10,000, and 15,000 bp; reference band at 3,000 bp has increased intensity for orientation; recommended volume: 5–10 μL per lane |
| Sensitivity / LOD | Detection limit: ~20 ng per band with ethidium bromide; ~5 ng per band with SYBR Safe or SYBR Gold |
| Specificity | All fragments are verified by restriction mapping and sequencing; no detectable nicked or supercoiled plasmid contamination; consistent electrophoretic migration from lot to lot |
| Reaction Conditions / Protocol | Load 5–10 μL of marker directly onto gel; no pre-heating required; run at 3–8 V/cm; for optimal separation of fragments >8 kb, use 0.5–0.8% agarose gels and lower voltages (1–3 V/cm) with extended run times |
| Components / Formulation | Purified double-stranded DNA restriction fragments in storage buffer (10 mM Tris-HCl pH 8.0, 1 mM EDTA, 5% Ficoll 400, bromophenol blue, xylene cyanol FF, orange G tracking dyes) |
| Storage Conditions | 2–8°C for up to 6 months; –20°C for long-term storage (>1 year); avoid exposure to nucleases and repeated pipetting from stock |
| Shelf Life | 24 months at –20°C; 6 months at 2–8°C |
| Package Specifications | 500 μL (50–100 applications) and 5 × 500 μL bulk pack |
| Product Form | Ready-to-use liquid; loading dyes included in formulation |
| Quality Control | Each lot validated: 15 discrete bands visible on 0.8% agarose gel; log-linear relationship established (R² ≥0.99); fragment sizes confirmed by Bioanalyzer capillary electrophoresis; no degradation products visible |
| Key Features | Broad molecular weight range extends to 15 kb; heavy reference band at 3 kb for orientation; combined tracking dye system for monitoring migration of both small and large fragments; applicable for both analytical and preparative gel applications; compatible with gel documentation and image analysis software |
For research use only, not for clinical use.
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