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| Product Name | 1 and 2.8 µm Oligo(dT) Magnetic Beads for High-Purity mRNA Isolation |
| Catalog No. | IRMGM-0054 |
| Description | Alta DiagnoTech Oligo(dT) magnetic beads carry covalently coupled oligo(dT) for complementary binding to the poly(A) tails of eukaryotic mRNA. The beads rapidly isolate intact, high-purity mRNA from total RNA or directly from animal and plant tissues or cell lysates. |
| Intended Use | Isolation of eukaryotic mRNA for RT-PCR, solid-phase cDNA library construction, RACE and Northern blot workflows. |
| Principle / Technology | Poly(A)-oligo(dT) complementary hybridization followed by magnetic separation. |
| Sample Type | Eukaryotic total RNA, animal tissue, plant tissue and cell lysate |
| Performance Range / Specifications | 1 or 2.8 µm particles at 5 mg/mL; oligo(dT) coupling of 500 pmol/mg for 1 µm beads and 400 pmol/mg for 2.8 µm beads; mRNA binding capacity 1-2 µg/mg beads. |
| Storage Conditions | 2-8 °C |
| Shelf Life | 2 years |
| Package Specifications | 1, 2, 5 or 100 mL |
| Product Form | Oligo(dT)-functionalized magnetic beads |
| Key Features | 1 and 2.8 µm particle options 1-2 µg mRNA binding per mg beads Direct tissue and cell-lysate compatibility Multiple downstream molecular applications |
| Concentration | 5 mg/mL |
| Product Type | mRNA capture magnetic beads |
| Function / Efficacy | Affinity isolation of intact eukaryotic mRNA. |
| Storage Buffer | 1× PBS containing 0.1% v/v ProClin 300 |
| Analytes / Targets | Polyadenylated eukaryotic mRNA |
| Matrix | Magnetic microspheres |
| Particle Size | 1 or 2.8 µm |
| Ligand / Bonded Phase | Oligo(dT) |
| Ligand Coupling Method | Covalent coupling to the bead surface |
For research use only, not for clinical use.
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