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| Product Name | 0.2-5 mL Circulating Cell-Free DNA Extraction Kit with >85% Recovery |
| Catalog No. | DREK-0141 |
| Description | Alta DiagnoTech circulating DNA kit uses superparamagnetic microspheres and premixed buffers to recover cell-free DNA from serum, plasma, urine and other cell-free body fluids. |
| Intended Use | Research extraction of circulating cell-free DNA for PCR, sequencing and analytical detection. |
| Principle / Technology | Magnetic capture of cell-free DNA using superparamagnetic microspheres and an optimized buffer system. |
| Detection Method | Manual or automated magnetic extraction followed by qPCR, PCR, sequencing or concentration measurement |
| Sample Type | 0.2-5 mL serum, plasma, urine and other cell-free body fluids |
| Performance Range / Specifications | Recovery exceeds 85% for the displayed 151 bp DNA fragment spiked into plasma at 10 ng/mL across 0.2-5 mL sample volumes. Elution volume can be as low as 20 µL. |
| Components / Formulation | Superparamagnetic microspheres and premixed extraction buffers |
| Package Specifications | 24 reactions for 1-4 mL samples; 100 reactions for 1 mL samples; 50 reactions for 4 mL samples; an additional automated 100-reaction, 1 mL format is listed |
| Product Form | Magnetic-bead circulating DNA extraction kit |
| Key Features | Flexible 0.2-5 mL input, >85% reference recovery, high-purity eluate, low 20 µL elution volume and manual or automated processing. |
| Compatibility | Manual processing, large-volume automated magnetic extraction, PCR, sequencing and analytical detection |
| Application Notes / Precautions | In repeated extraction of plasma spiked with a 151 bp DNA fragment, qPCR Ct values were consistent across runs. |
| Product Type | Circulating cell-free DNA extraction kit |
| Function / Efficacy | Recover cell-free DNA from serum, plasma, urine and related cell-free fluids. |
| Analytes / Targets | Circulating cell-free DNA |
For research use only, not for clinical use.
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