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| Product Name | Ziehl-Neelsen Acid-Fast Stain Kit, for Mycobacteria Detection |
| Catalog No. | ATRSA-0009 |
| Description | A heat-based acid-fast staining kit using carbol fuchsin, acid alcohol decolorizer, and methylene blue counterstain for the microscopic detection of Mycobacterium species and other acid-fast organisms. |
| Intended Use | Detection of Mycobacterium tuberculosis and other acid-fast bacilli in clinical sputum smears, tissue sections, and culture isolates for tuberculosis diagnosis and non-tuberculous mycobacteria identification. |
| Principle / Technology | Carbol fuchsin with phenol penetrates the mycolic acid-rich waxy cell wall of mycobacteria upon heating; acid alcohol fails to decolorize mycobacteria due to their lipid-rich wall; methylene blue counterstains non-acid-fast background cells. |
| Detection Method | Bright-field light microscopy at 100x oil immersion; fluorescence option available with auramine-rhodamine variant |
| Sample Type | Sputum smears, bronchoalveolar lavage, tissue biopsies, concentrated clinical specimens, culture isolates |
| Performance Range / Specifications | Detection sensitivity ~5,000–10,000 AFB/mL of sputum by direct smear |
| Sensitivity / LOD | ~5,000–10,000 organisms per mL sputum by direct smear microscopy |
| Specificity | Acid-fast positive: Mycobacteria, Nocardia (partial), Rhodococcus, Cryptosporidium oocysts; non-acid-fast: all other bacteria |
| Reaction Conditions / Protocol | Flood carbol fuchsin onto fixed smear; heat slide until steaming for 5 min (add stain if drying); cool; decolorize with acid alcohol; rinse; counterstain with methylene blue 1–2 min; rinse; air dry. |
| Components / Formulation | Carbol Fuchsin (basic fuchsin in phenol), Acid Alcohol 3% HCl in 95% ethanol, Methylene Blue 0.3% counterstain |
| Storage Conditions | 15–30°C, flammable, tightly sealed, protect from light |
| Shelf Life | 24 months |
| Package Specifications | Kit for 100 tests, 250 tests |
| Product Form | Liquid reagents in dropper bottles |
| Quality Control | AFB staining verification with M. tuberculosis H37Ra and E. coli (negative control), staining intensity |
| Key Features | Complete kit; standard for tuberculosis diagnosis; validated with reference mycobacterial strains; ready to use |
For research use only, not for clinical use.
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