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| Product Name | DAF-2 DA Nitric Oxide Fluorescent Probe |
| Catalog No. | RESK-0561 |
| Description | DAF-2 DA (4,5-diaminofluorescein diacetate), a 4,5-diaminofluorescein derivative, is a fluorescent probe for the detection of nitric oxide (NO). The probe is cell permeant; after entering cells it is hydrolyzed by intracellular esterases to DAF-2, which cannot cross the cell membrane. DAF-2 itself is only weakly fluorescent, but in the presence of oxygen it reacts with NO to generate the strongly fluorescent triazolofluorescein DAF-2T, with an excitation wavelength of 491 nm and an emission wavelength of 513 nm. DAF-2 DA is a relatively sensitive fluorescent NO probe, with a detection limit of 2-5 nM at neutral pH. |
| Intended Use | For fluorescence-based detection of nitric oxide (NO) in cells. |
| Principle / Technology | Cell-permeant DAF-2 DA is converted by intracellular esterases into membrane-impermeant DAF-2. In the presence of oxygen, DAF-2 reacts with NO to form the highly fluorescent triazolofluorescein DAF-2T (Ex 491 nm, Em 513 nm), and fluorescence intensity reflects the NO level. |
| Detection Method | Fluorescence detection at Ex 491 nm / Em 513 nm using fluorescence microscopy, laser scanning confocal microscopy, flow cytometry, or a microplate reader. |
| Sample Type | Cells (intracellular NO detection). |
| Performance Range / Specifications | Synonym: 4,5-Diaminofluorescein diacetate CAS No.: 205391-02-2 Molecular formula: C24H18N2O7 Molecular weight: 446.41 Ex/Em: 491/513 nm Solubility: readily soluble in DMSO (≥5 mM) Supplied concentration: 1 mM (100 μg dissolved in 200 μL DMSO) |
| Sensitivity / LOD | NO detection limit of 2-5 nM at neutral pH. |
| Specificity | Reacts with nitric oxide in the presence of oxygen to form the strongly fluorescent triazolofluorescein DAF-2T. |
| Reaction Conditions / Protocol | Dissolve 100 μg of DAF-2 DA in 200 μL of DMSO to obtain a 1 mM solution. Dilute as required, load into cells, and measure fluorescence at Ex 491 nm / Em 513 nm. |
| Components / Formulation | Single-component reagent supplied as a 1 mM solution in DMSO (100 μg in 200 μL). |
| Storage Conditions | Store at -20 °C protected from light; avoid repeated freeze-thaw cycles. |
| Shelf Life | 1 year when stored at -20 °C protected from light. |
| Package Specifications | 100 μg (supplied as a 1 mM solution in 200 μL DMSO) |
| Product Form | Solution in DMSO (1 mM, 200 μL). |
| Concentration | 1 mM (100 μg dissolved in 200 μL DMSO). |
| Molecular Weight | 446.41 |
| pH Range / Optimal pH | Detection limit of 2-5 nM stated at neutral pH. |
| Shipping Conditions | Shipped with ice packs. |
| Expiration Date / Stability | Stable for 1 year at -20 °C protected from light; avoid repeated freeze-thaw cycles. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Compatible with fluorescence microscopes, laser scanning confocal microscopes, flow cytometers, and microplate readers. |
| Recommended Buffer System | DMSO for stock solution preparation (soluble at ≥5 mM). |
| Application Notes / Precautions | Protect from light and avoid repeated freeze-thaw cycles. The product is supplied as a liquid at 1 mM in 200 μL DMSO. |
For research use only, not for clinical use.
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