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| Product Name | Coomassie Brilliant Blue R-250 Protein Gel Stain, 0.1% Solution |
| Catalog No. | ATRSA-0004 |
| Description | A ready-to-use colloidal Coomassie Brilliant Blue R-250 staining solution for the visualization of proteins separated by SDS-PAGE with detection sensitivity down to 8–10 nanograms per band. |
| Intended Use | Detection of total protein bands in polyacrylamide gels following SDS-PAGE or native PAGE for purity assessment, molecular weight determination, and Western blot loading control visualization. |
| Principle / Technology | Coomassie R-250 dye binds to basic amino acid residues (arginine, lysine, histidine) and aromatic residues through electrostatic and hydrophobic interactions, forming a blue protein-dye complex visible in the gel matrix. |
| Detection Method | Densitometric analysis of stained gels at 595 nm, visual inspection against white light illuminator |
| Sample Type | Proteins separated on polyacrylamide gels (SDS-PAGE, Native PAGE, IEF gels) |
| Performance Range / Specifications | Linear dynamic range 10–2000 ng/band; detection limit ~8–10 ng BSA per band |
| Sensitivity / LOD | 8–10 ng per band for BSA standard |
| Specificity | Stains all protein types; also stains some glycoproteins and lipoproteins with reduced sensitivity |
| Reaction Conditions / Protocol | Fix gel in 40% methanol, 10% acetic acid for 30 min; stain with Coomassie R-250 for 1–2 hours with gentle agitation; destain in 40% methanol, 10% acetic acid until background is clear. |
| Components / Formulation | Coomassie Brilliant Blue R-250 0.1% in 50% methanol, 10% acetic acid |
| Storage Conditions | 15–30°C, tightly sealed to prevent evaporation |
| Shelf Life | 24 months |
| Package Specifications | 500 mL, 1 L |
| Product Form | Liquid, ready to use |
| Quality Control | Sensitivity verification with BSA standard dilution series, staining uniformity within gel |
| Key Features | Ready to use; consistent staining intensity; compatible with mass spectrometry after destaining; economical; standard for protein gel visualization |
For research use only, not for clinical use.
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