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| Product Name | Nuclear Yellow (Hoechst S769121) Neuronal Tracer Dye |
| Catalog No. | RESK-0551 |
| Description | Nuclear Yellow (Hoechst S769121) is a long-wavelength neuronal tracer widely used in neuroscience research. It specifically labels neuronal nuclei with yellow fluorescence and is commonly combined with the retrograde tracer True Blue, a UV-excitable divalent cationic dye that labels the cytoplasm with blue fluorescence, as a classic dual-color labeling pair. Both tracers withstand immunohistochemical processing and can photoconvert DAB into an insoluble electron-dense product, making them suitable for electron microscopy studies. |
| Intended Use | For fluorescent labeling and tracing of neuronal nuclei in neuroanatomical and neurobiological research, including dual-labeling paradigms and light/electron microscopy studies. |
| Principle / Technology | Nuclear Yellow is a long-wavelength fluorescent tracer that binds to neuronal nuclei and emits yellow fluorescence. Under illumination it can photoconvert diaminobenzidine (DAB) into an insoluble electron-dense reaction product, enabling correlative electron microscopy. |
| Detection Method | Fluorescence microscopy; Ex/Em 372/506 nm; compatible with DAB photoconversion for electron microscopy. |
| Sample Type | Neuronal tissue and cells |
| Performance Range / Specifications | Appearance: Solid CAS NO.: 74681-68-8 Formula: C25H25N7O2S·3ClH Molecular Weight: 596.96 Ex/Em: 372/506 nm Solubility: Soluble in DMSO |
| Storage Conditions | Store at -25 to -15 °C, protected from light. |
| Shelf Life | 1 year |
| Package Specifications | 5 mg, 10 mg |
| Product Form | Solid |
| Molecular Weight | 596.96 |
| Expiration Date / Stability | Stable for 1 year when stored at -25 to -15 °C protected from light. |
| Regulatory / Compliance | For research use only. |
| Application Notes / Precautions | Protect from light. Dissolve in DMSO. Compatible with immunohistochemical processing and DAB photoconversion for electron microscopy. |
For research use only, not for clinical use.
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