| Product Name | MTT Cell Proliferation Assay Reagent |
| Catalog No. | CATR-HMM-0028 |
| Description | Methylthiazolyldiphenyl-tetrazolium bromide (MTT), also known as bromothymol blue tetrazolium, is a yellow dye that has widely replaced traditional trypan blue staining or radioactive isotope insertion methods for detecting cell viability, cell proliferation, and cytotoxicity analysis. The detection principle is as follows: MTT is a compound dye that can accept hydrogen ions, carries a positive charge, and has cell membrane permeability. The succinate dehydrogenase in the mitochondria of live cells can reduce the externally introduced MTT into water-insoluble deep blue MTT-methylene blue crystals, while dead cells lack this function. MTT crystals cannot penetrate the cell membrane and thus accumulate within proliferating, undamaged cells. These crystals can be dissolved using DMSO or acidified isopropanol, and the absorbance at 570 nm measured by a microplate reader reflects the amount of MTT crystals formed. The amount of MTT crystals formed is directly proportional to the number of live cells, thereby assessing cell viability. |
| Application | This product can be used to detect cell viability, cell proliferation, and cell toxicity analysis. |
| Applicable Instruments | Fluorescence microscope, flow cytometer |
| Storage | Store at 4°C. |
| Shelf Life | 1 year |
| Features | This assay kit uses MTT (high purity) as an indicator. Based on the classic operational steps, it employs a unique methylene blue dissolution solution formulation that can directly dissolve methylene blue precipitates without the need to remove the original culture medium. This avoids operational errors caused by partial removal of methylene blue during the removal of the culture medium. It features low background, high sensitivity, a wide linear range, and simple operation. Additionally, this kit includes disposable needle filters and syringes, providing greater convenience for experiments. |
For research use only, not for clinical use.
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