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| Product Name | Wright's Stain Solution, for Blood Smear Differential |
| Catalog No. | ATRSA-0002 |
| Description | A buffered Wright's methanolic staining solution for the differentiation of white blood cells in peripheral blood and bone marrow smears, providing characteristic cytoplasmic granule and nuclear chromatin staining. |
| Intended Use | Differential leukocyte count in peripheral blood smear examination; morphological evaluation of red blood cells, white blood cells, and platelets in hematology laboratories. |
| Principle / Technology | Polychrome methylene blue and eosin Y form complexes that stain acidic cellular components blue (basophilic granules, nuclei) and basic components orange-pink (eosinophilic granules, hemoglobin); methanol acts as fixative and solvent. |
| Detection Method | Light microscopy at 50x–100x oil immersion for WBC differential count |
| Sample Type | Peripheral blood smears, bone marrow aspirate smears, buffy coat preparations |
| Performance Range / Specifications | Five-part WBC differential achievable: neutrophils, lymphocytes, monocytes, eosinophils, basophils clearly distinguishable |
| Sensitivity / LOD | N/A |
| Specificity | Granulocyte granules (neutrophilic, eosinophilic, basophilic) characteristically stained for lineage identification |
| Reaction Conditions / Protocol | Cover smear with Wright's stain for 1–3 min (methanol fixation); add equal volume of phosphate buffer pH 6.8; mix gently; stain 3–5 min; rinse with buffer; air dry. |
| Components / Formulation | Polychrome methylene blue, eosin Y in methanol, phosphate buffer not included |
| Storage Conditions | 15–30°C, flammable, tightly sealed to exclude moisture |
| Shelf Life | 24 months |
| Package Specifications | 500 mL, 1 L |
| Product Form | Liquid, ready to use |
| Quality Control | Staining intensity validation with normal blood smear reference slides, lot-to-lot comparison |
| Key Features | Ready to use; methanol-based single-step fixation and staining; standard in hematology; distinctive granule staining |
For research use only, not for clinical use.
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