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| Product Name | Antibody Diluent Solution, Low Background |
| Catalog No. | ATRD-0003 |
| Description | A protein-stabilized diluent specifically designed for the dilution of primary and secondary antibodies in Western blotting, ELISA, and immunohistochemistry, reducing non-specific binding while preserving antibody reactivity. |
| Intended Use | Diluting primary antibodies and HRP- or AP-conjugated secondary antibodies for use in membrane-based and plate-based immunoassays with minimal background signal. |
| Principle / Technology | Bovine serum albumin or casein as carrier protein saturates non-specific binding sites; non-ionic surfactant Tween 20 reduces hydrophobic interactions; buffer maintains optimal pH for antibody-antigen binding. |
| Detection Method | Western blot signal-to-noise ratio analysis, ELISA zero-standard optical density measurement |
| Sample Type | Polyclonal and monoclonal primary antibodies, species-specific secondary antibody conjugates |
| Performance Range / Specifications | Background OD <0.1 in ELISA; signal-to-noise ratio >10:1 in Western blot |
| Sensitivity / LOD | N/A |
| Specificity | No detectable reactivity with common secondary antibody species (anti-mouse, anti-rabbit, anti-goat) |
| Reaction Conditions / Protocol | Use undiluted; dilute antibodies according to manufacturer or optimized protocol; prepare fresh for each use. |
| Components / Formulation | Tris-buffered saline pH 7.4, BSA (IgG-free), Tween 20, ProClin 300 |
| Storage Conditions | 2–8°C |
| Shelf Life | 12 months |
| Package Specifications | 100 mL, 500 mL |
| Product Form | Liquid, ready to use |
| Quality Control | Background and sensitivity verification with reference antibodies, preservative efficacy |
| Key Features | Ready to use; low background formulation; preserves antibody activity overnight at 4°C; IgG-free BSA |
For research use only, not for clinical use.
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