In recent years, the demand for accurate and sensitive drug detection in biological samples has surged across multiple scientific research fields, including pharmacology, toxicology, clinical pharmacokinetics, and forensic toxicology. Researchers and laboratory professionals often face challenges in quantifying low-concentration drug components in complex biological matrices—such as whole blood, serum, and plasma—where interfering substances (e.g., proteins, lipids, endogenous metabolites) can obscure target signals, leading to inaccurate or unreliable results. Traditional detection methods, such as basic immunoassays or early-generation chromatographic techniques, often fail to meet the rigorous requirements of modern research: many lack the sensitivity to detect drugs at concentrations below 20 ng/mL, require cumbersome sample pretreatment steps (e.g., multiple extraction or purification cycles), or are limited to a single type of biological sample, restricting experimental flexibility.
Against this backdrop, the development of specialized assay kits tailored for multi-sample-type drug detection has become a critical focus. Our Whole Blood, Serum, Plasma Single Drug Assay Kit (Cat.No: CD-00142) addresses these industry pain points by leveraging advanced bioanalytical technologies. Unlike conventional kits that may only support serum or plasma testing, this product is optimized to handle three common biological sample types—whole blood, serum, and plasma—eliminating the need for researchers to purchase separate kits for different samples and reducing experimental costs and workflow complexity.
Key drivers behind the kit’s development include:
Growing demand for low-level drug quantification: In pharmacokinetic studies, tracking drug concentrations in vivo often requires detecting trace amounts (10–50 ng/mL) of drugs to analyze absorption, distribution, metabolism, and excretion (ADME) profiles. This kit’s 15 ng/mL cut-off and detection limit directly meet this need, ensuring no missed data points for low-concentration drug samples.
Need for simplified workflows: Traditional drug assay protocols may involve 2–3 hours of sample preparation (e.g., protein precipitation, solid-phase extraction) before detection. Our kit streamlines this process with pre-optimized reagents and step-by-step instructions, minimizing hands-on time and reducing the risk of human error.
Versatility for cross-field research: Whether used in academic laboratories studying drug-drug interactions, pharmaceutical R&D teams evaluating new drug candidates, or forensic research investigating drug-related cases, the kit’s compatibility with multiple sample types makes it a flexible tool for diverse research objectives.
Regulatory and reproducibility requirements: Modern scientific research emphasizes data reproducibility and compliance with standard operating procedures (SOPs). This kit undergoes rigorous quality control (QC) testing—including batch-to-batch consistency checks, sensitivity validation, and specificity verification—to ensure stable performance across different experiments and laboratories.
Broad Sample Compatibility: Specifically designed to work with whole blood, serum, and plasma samples. It eliminates the need for sample type-specific preprocessing (e.g., additional lysis steps for whole blood or protein depletion for plasma), allowing direct use of minimally treated samples and saving 1–2 hours of sample preparation time per experiment.
Ultra-Sensitive Detection Performance: Boasts a detection limit and cut-off of 15 ng/mL, outperforming many conventional kits (which typically have cut-offs of 20–50 ng/mL). This sensitivity enables accurate quantification of trace drug residues in biological samples, critical for studies involving low-dose drug administration or late-stage drug metabolism.
Robust Anti-Interference Capability: Incorporates a proprietary reagent formulation that reduces matrix interference from biological components (e.g., hemoglobin in whole blood, albumin in serum, clotting factors in plasma). Validation tests show that the kit maintains ≥95% accuracy even in samples with high protein content (e.g., plasma with total protein levels of 60–80 g/L) or lipid-rich whole blood.
User-Friendly Operation: Requires no specialized technical expertise or advanced laboratory equipment (e.g., high-performance liquid chromatography (HPLC) or mass spectrometry (MS) systems). The kit includes pre-mixed reagents, clear step-by-step protocols, and a simplified reaction system—researchers can complete the entire assay (from sample addition to result readout) in approximately 60–90 minutes.
Stable Storage and Transportation: Formulated with lyophilized key reagents and a buffer system that maintains activity at 4–30°C. This stable storage range eliminates the need for expensive cold-chain transportation (e.g., dry ice) and allows long-term storage in standard laboratory refrigerators or ambient temperature cabinets (when unopened), reducing logistics costs and storage constraints for laboratories.
Cost-Effective for Multi-Sample Research: By supporting whole blood, serum, and plasma testing in one kit, it eliminates the expense of purchasing three separate kits for different sample types. For a typical laboratory conducting 50–100 tests per month, this can reduce annual reagent costs by 30–40% compared to single-sample-type alternatives.
High Data Accuracy and Reproducibility: Undergoes strict QC testing for each production batch, including:
Sensitivity verification: Consistent detection of 15 ng/mL standard samples with a coefficient of variation (CV) ≤5%.
Specificity testing: No cross-reactivity with 20+ common endogenous substances (e.g., bilirubin, glucose, common antibiotics) or structurally similar drugs.
Reproducibility checks: Inter-assay and intra-assay CVs ≤8%, ensuring results are reliable across different experiments and operators.
Time-Saving Workflow Integration: The simplified sample handling process (no complex extraction or purification) and fast reaction time (60–90 minutes total) align with the high-throughput needs of modern laboratories. For example, a team studying drug metabolism can process 25 samples (the kit’s 25 T format) in a single workday, compared to 10–15 samples with traditional methods.
Flexible Application Scope: Suitable for a wide range of research scenarios, including:
Pharmacokinetic studies: Tracking drug concentration changes in animal or human biological samples over time.
Toxicology research: Evaluating drug toxicity by quantifying drug accumulation in target tissues via blood samples.
Preclinical drug development: Assessing drug bioavailability in animal models.