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| Product Name | Western Blot Chemiluminescence Detection Substrate Kit |
| Catalog No. | PTR-HMM-0057 |
| Description | An enhanced chemiluminescence (ECL) substrate kit for the sensitive detection of horseradish peroxidase (HRP)-conjugated antibodies in Western blotting applications. The two-component system produces an intense, long-lasting luminescent signal. |
| Intended Use | Detection of HRP-labeled secondary antibodies in Western blot membrane-based immunodetection of target proteins extracted from cells or tissues. |
| Principle / Technology | HRP catalyzes the oxidation of luminol in the presence of hydrogen peroxide to produce an excited-state intermediate that emits light at 425 nm upon decay to the ground state. |
| Detection Method | Chemiluminescence detection using X-ray film, CCD camera, or digital imaging system |
| Sample Type | PVDF or nitrocellulose membrane with immobilized proteins and HRP-conjugated antibodies |
| Performance Range / Specifications | Detection sensitivity: low-picogram to high-femtogram range of target protein, depending on antibody affinity |
| Sensitivity / LOD | ≤1 pg of target protein (with optimized primary and secondary antibodies) |
| Specificity | Signal generation is dependent on the presence of HRP enzyme; negligible non-specific background when proper blocking and washing steps are performed |
| Reaction Conditions / Protocol | Mix equal volumes of luminol/enhancer solution and peroxide solution, apply to membrane, incubate for 1–5 minutes, drain excess reagent, expose to film or imaging system |
| Components / Formulation | Solution A: Luminol with enhancer compound, Solution B: Stabilized hydrogen peroxide solution, provided in two separate bottles for mixing before use |
| Storage Conditions | 2–8°C protected from light; working solution should be used within 30 minutes of preparation |
| Shelf Life | 12 months from date of manufacture |
| Package Specifications | 500 mL total (250 mL Solution A + 250 mL Solution B), sufficient for approximately 5,000 cm² of membrane |
| Product Form | Two-component liquid reagent system |
| Quality Control | Each lot tested for signal intensity, duration (>6 hours signal stability), and background levels using standardized Western blot system; endotoxin tested |
| Key Features | Extended signal duration allows multiple exposures without signal decay; picogram-level sensitivity; compatible with all commercial HRP-conjugated secondary antibodies; low background formulation |
For research use only, not for clinical use.
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