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| Product Name | Bradford Protein Assay Reagent (Coomassie Brilliant Blue G-250) |
| Catalog No. | PTR-HMM-0056 |
| Description | A rapid and simple colorimetric protein assay reagent based on the Coomassie Brilliant Blue G-250 dye-binding method. The reagent is supplied as a ready-to-use solution that changes color from brown to blue upon binding to protein. |
| Intended Use | Rapid protein concentration determination in biological samples including cell extracts, tissue homogenates, and purified protein solutions for research applications. |
| Principle / Technology | Binding of Coomassie Brilliant Blue G-250 dye to basic and aromatic amino acid residues in proteins causes a shift in the dye's absorbance maximum from 465 nm to 595 nm, with the color change proportional to protein concentration. |
| Detection Method | Colorimetric measurement at 595 nm |
| Sample Type | Cell lysates, tissue extracts, purified proteins, column chromatography fractions; compatible with reducing agents |
| Performance Range / Specifications | Linear range: 1–25 μg/mL (standard protocol); 0.5–10 μg/mL (micro protocol) |
| Sensitivity / LOD | ≤1 μg/mL |
| Specificity | Detects most proteins with varying sensitivity depending on amino acid composition; arginine and aromatic residues produce stronger responses |
| Reaction Conditions / Protocol | Add reagent to sample at room temperature, mix gently, incubate for 5–10 minutes, measure absorbance at 595 nm |
| Components / Formulation | Ready-to-use Coomassie Brilliant Blue G-250 reagent in phosphoric acid and ethanol, BSA standard (2 mg/mL), detailed protocol with microplate and cuvette instructions |
| Storage Conditions | 2–8°C protected from light; reagent is stable at room temperature for short-term use during experiments |
| Shelf Life | 24 months |
| Package Specifications | 950 mL (3,800 standard assays in 5 mL format) |
| Product Form | Ready-to-use liquid solution |
| Quality Control | Each lot validated with BSA standard curve (R2 ≥0.99); tested for compatibility with common buffers and detergents |
| Key Features | Single-reagent format requires no mixing or preparation steps; results in 5 minutes; compatible with standard spectrophotometers and microplate readers; minimal interference from Tris, EDTA, and reducing agents |
For research use only, not for clinical use.
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