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| Product Name | Silver Stain Protein Gel Detection Kit |
| Catalog No. | PTR-HMM-0061 |
| Description | An ultrasensitive silver staining kit for the detection of proteins separated by SDS-PAGE. This kit provides sensitivity superior to conventional Coomassie staining while maintaining a simplified protocol suitable for routine laboratory use. |
| Intended Use | High-sensitivity detection of proteins in polyacrylamide gels following SDS-PAGE, suitable for samples with low protein abundance or when maximum detection sensitivity is required. |
| Principle / Technology | Silver ions bind to protein functional groups and are reduced to metallic silver under controlled conditions, forming visible brown-black bands proportional to protein concentration. |
| Detection Method | Visual detection of protein bands on polyacrylamide gel; compatible with image documentation systems |
| Sample Type | Proteins separated in polyacrylamide gels (0.5–1.5 mm thickness); compatible with Tris-glycine, Tris-tricine, and Bis-Tris gel systems |
| Performance Range / Specifications | Detection range: 0.25–5 ng per band for most proteins; linear quantitation range over one order of magnitude |
| Sensitivity / LOD | ≤0.25 ng of protein per band (approximately 10–50× more sensitive than Coomassie Brilliant Blue R-250) |
| Specificity | Stains most proteins including glycoproteins and lipoproteins; some highly acidic proteins may stain less intensely |
| Reaction Conditions / Protocol | Fix gel (30 min) → wash (2x10 min) → sensitize (1 min) → wash (2x1 min) → silver impregnate (20 min) → wash (1 min) → develop (2–10 min) → stop; total protocol approximately 90 minutes |
| Components / Formulation | Fixing solution concentrate, sensitizing solution, silver nitrate solution, developer concentrate, stop solution, detailed protocol with troubleshooting guide |
| Storage Conditions | 2–8°C; silver nitrate solution must be protected from light; developer should be prepared fresh immediately before use |
| Shelf Life | 12 months from date of manufacture |
| Package Specifications | Sufficient reagents for staining 25 mini-gels (8 x 10 cm) |
| Product Form | Liquid reagent concentrates |
| Quality Control | Each lot tested with standardized protein mixtures (0.5 ng–500 ng per band); sensitivity verified against Coomassie-stained reference; background staining evaluated on blank gel lanes |
| Key Features | Mass spectrometry-compatible protocol option available; approximately 50× greater sensitivity than Coomassie R-250; compatible with subsequent Western blot transfer analysis |
For research use only, not for clinical use.
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