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| Product Name | Ultra-Sensitive ECL Substrate Kit (Femtogram Detection) |
| Catalog No. | PTR-HMM-0084 |
| Description | An enhanced chemiluminescent substrate system for horseradish peroxidase detection in Western blotting, delivering femtogram-level detection sensitivity. The two-component formulation generates an intense and prolonged luminescent signal upon reaction with HRP-conjugated secondary antibodies. The extended signal half-life exceeding 6 hours facilitates multiple exposures and digital imaging optimization without signal decay. The substrate is compatible with both PVDF and nitrocellulose membranes. |
| Intended Use | High-sensitivity chemiluminescent detection of HRP-labeled probes on immunoblots for low-abundance protein visualization. |
| Principle / Technology | HRP-catalyzed luminol oxidation with enhanced chemiluminescence |
| Detection Method | Chemiluminescence imaging (CCD or X-ray film) |
| Sample Type | Proteins transferred to PVDF or nitrocellulose membrane |
| Performance Range / Specifications | Detection limit: as low as 1 fg of target protein; signal half-life: ≥6 hours |
| Sensitivity / LOD | Femtogram-level detection comparable to radioactive methods without associated hazards |
| Specificity | Specific signal proportional to HRP-conjugated secondary antibody bound to primary antibody-target protein complex |
| Reaction Conditions / Protocol | Mix equal volumes of Peroxide Solution and Luminol/Enhancer Solution immediately before use; incubate membrane 1–2 min at room temperature; drain excess substrate before imaging |
| Components / Formulation | Luminol/Enhancer Solution (50 mL), Stable Peroxide Solution (50 mL) |
| Storage Conditions | 2–8 °C, protected from light |
| Shelf Life | 12 months |
| Package Specifications | 100 mL total (sufficient for approximately 1000 cm² membrane area) |
| Product Form | Two liquid reagent bottles |
| Quality Control | Each lot tested by serial dilution of an HRP-labeled protein standard; detection sensitivity verified to ≤10 fg |
| Key Features | Extended signal duration eliminates timing pressure during imaging; longer half-life supports film and digital detection methods equally |
| Purity | As specified per product type; recombinant proteins ≥90% by SDS-PAGE |
| Concentration | As specified on product label or certificate of analysis |
| Activity / Unit Definition | Binding capacity or enzymatic activity as specified per product documentation |
| Molecular Weight | Varies by product as specified in documentation |
| Source / Origin | Recombinant expression in E. coli, mammalian, or insect cell systems; synthetic chemistry for small molecule reagents |
| pH Range / Optimal pH | pH 7.0–8.0 for most protein applications |
| Shipping Conditions | Ambient for dry reagents; cold packs for liquid protein solutions; dry ice for frozen products |
| Expiration Date / Stability | 12–24 months at recommended storage temperature |
| Regulatory / Compliance | Research use; manufactured under ISO 9001 quality system |
| Compatibility | Compatible with standard SDS-PAGE, Western blot, mass spectrometry, and chromatography systems |
| Recommended Buffer System | PBS, Tris, or HEPES buffers as specified in product protocol |
| Application Notes / Precautions | Avoid repeated freeze-thaw cycles for protein solutions; use fresh aliquots; protect light-sensitive reagents |
| Batch-to-Batch Consistency | Protein concentration and activity within ±15% of reference lot |
For research use only, not for clinical use.
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