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| Product Name | Trypan Blue Staining Solution, 0.2% |
| Catalog No. | RERE-0294 |
| Description | Mid-strength trypan blue solution at 0.2 percent for routine cell staining and counting. The dye exclusion principle applies across concentrations: intact membranes of living cells keep the anionic dye out while damaged membranes of dead cells admit it, so blue cells are scored as non-viable during hemocytometer or automated counts. The reagent keeps for 12 months at room temperature. |
| Intended Use | Viability assessment by dye exclusion during cell counting |
| Principle / Technology | Dead cells with compromised membranes take up trypan blue and appear blue, while live cells exclude the dye |
| Specificity | 0.2 percent; 12 month shelf life |
| Reaction Conditions / Protocol | Mix an aliquot of the cell suspension with the solution, load the counting chamber and score stained versus unstained cells |
| Storage Conditions | Room temperature |
| Package Specifications | 10 mL |
| Key Features | Intermediate strength suited to standard counting dilutions; simple dye-exclusion readout; 12 month shelf life |
For research use only, not for clinical use.
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