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| Product Name | TE Buffer (Tris-EDTA, pH 8.0), Molecular Biology Grade |
| Catalog No. | NATR-HMM-0125 |
| Description | A ready-to-use molecular biology grade Tris-EDTA buffer solution at pH 8.0, prepared with ultra-pure components and nuclease-free water. TE buffer is the standard diluent and storage solution for DNA and RNA in molecular biology applications. |
| Intended Use | Resuspension and storage of purified DNA and RNA; dilution buffer for nucleic acid preparations; elution of DNA from silica membrane columns; preparation of enzymatic reaction mixtures; storage of oligonucleotide primers and probes; and as a general-purpose nucleic acid buffer. |
| Principle / Technology | Tris (tris(hydroxymethyl)aminomethane) provides pH buffering capacity at pH 8.0, maintaining the stability of nucleic acid phosphodiester bonds. EDTA (ethylenediaminetetraacetic acid) chelates divalent cations (Mg2+, Ca2+, Mn2+) that serve as essential cofactors for nucleases, thereby inhibiting DNase and RNase activities that could degrade stored nucleic acids. |
| Detection Method | pH measurement with calibrated electrode; conductivity measurement; DNase/RNase activity testing; UV spectrophotometry to verify absence of UV-absorbing contaminants; PCR inhibition testing |
| Sample Type | Storage buffer for genomic DNA, plasmid DNA, and PCR products; diluent for nucleic acid standards and samples; elution buffer for DNA purification protocols; reconstitution medium for lyophilized oligonucleotides |
| Performance Range / Specifications | Composition: 10 mM Tris-HCl, 1 mM EDTA, pH 8.0 ± 0.1 at 25°C; DNase-free; RNase-free; protease-free; sterile filtered (0.22 μm); endotoxin: ≤0.01 EU/mL |
| Sensitivity / LOD | No nucleic acid degradation detectable after incubation of 1 μg DNA in TE at 37°C for 24 hours; no detectable DNase or RNase activity by fluorescence-based nuclease assay |
| Specificity | No detectable nucleases; no phosphatase activity that would affect 5ʹ-phosphorylated DNA substrates; zero absorbance at 260 and 280 nm; no PCR inhibitors detectable in functional assays |
| Reaction Conditions / Protocol | Ready to use; pipette directly from bottle; use within 1 year of opening for highest quality; for long-term storage of DNA, store samples at –20°C or –80°C; TE is compatible with all common molecular biology procedures at up to 10% (v/v) in reaction mixtures |
| Components / Formulation | 10 mM Tris base, 1 mM EDTA disodium salt dihydrate, adjusted to pH 8.0 with HCl, dissolved in nuclease-free, deionized water (≥18.2 MΩ·cm), sterile filtered through 0.22 μm membrane |
| Storage Conditions | Room temperature (15–25°C); tightly capped; protect from contamination — use sterile technique when aliquoting; sterile-filtered bottles reduce contamination risk |
| Shelf Life | 36 months from date of manufacture in unopened container |
| Package Specifications | 100 mL, 500 mL, and 1 L sterile bottles |
| Product Form | Sterile-filtered, ready-to-use liquid |
| Quality Control | Each lot verified: pH 8.0 ± 0.05; DNase and RNase testing negative after 16-hour incubation; no detectable protease activity; endotoxin ≤0.01 EU/mL; conductivity within specification; sterility confirmed; no PCR inhibition up to 20% TE in reaction volume |
| Key Features | Pre-formulated and quality-tested, eliminating buffer preparation errors; nuclease-free certified for sensitive DNA/RNA work; Tris-EDTA combination provides long-term DNA stability without degradation; standard concentration compatible with all downstream enzymatic reactions; validated for qPCR and sequencing quality applications |
For research use only, not for clinical use.
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