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| Product Name | Silica-Membrane Genomic DNA Purification Kit |
| Catalog No. | NATR-HMM-0108 |
| Description | A silica membrane-based spin column kit for the rapid isolation of high-molecular-weight genomic DNA from a variety of biological starting materials. The chaotropic salt-based binding chemistry ensures efficient DNA capture and thorough removal of proteins and other contaminants, yielding DNA suitable for downstream enzymatic applications. |
| Intended Use | Purification of genomic DNA from whole blood, buffy coat, cultured cells, animal tissues, bacteria, and yeast for use in PCR, qPCR, Southern blotting, restriction digestion, and next-generation sequencing library preparation. |
| Principle / Technology | Chaotropic salts denature proteins and promote selective binding of DNA to the silica membrane in the spin column. After washing away contaminants with ethanol-containing buffers, purified DNA is eluted in low-salt buffer or nuclease-free water under mildly alkaline conditions. |
| Detection Method | UV spectrophotometry at 260/280 nm for purity assessment; agarose gel electrophoresis for integrity verification; fluorometric quantitation for precise concentration measurement |
| Sample Type | Whole blood (fresh or frozen, EDTA/citrate/heparin anticoagulated), buffy coat, cultured mammalian and bacterial cells, fresh or frozen animal tissues, yeast cells |
| Performance Range / Specifications | DNA yield from 200 μL whole blood: 4–12 μg; from 10^6 cultured cells: 15–25 μg; from 25 mg tissue: 10–35 μg; A260/A280 ratio: 1.7–1.9 |
| Sensitivity / LOD | Recovers genomic DNA from as few as 100 cells with micro-elution protocol |
| Specificity | Purified DNA is free of co-purified RNA; minimal bacterial DNA contamination with appropriate pretreatment; no detectable DNase or RNase activity in eluted DNA |
| Reaction Conditions / Protocol | Lyse sample in lysis buffer with proteinase K at 56°C for 10–30 minutes, add binding buffer with ethanol, transfer to spin column, centrifuge at 10,000g for 1 minute, wash twice with wash buffer, centrifuge dry, elute with 50–200 μL elution buffer at room temperature or 56°C; total protocol time: 30–60 minutes |
| Components / Formulation | Lysis buffer (guanidine hydrochloride, Tris-HCl, EDTA, SDS), binding buffer (guanidine hydrochloride, isopropanol), proteinase K solution (20 mg/mL), wash buffer concentrate (Tris-HCl, NaCl, EDTA, ethanol), elution buffer (10 mM Tris-HCl pH 8.5, 0.1 mM EDTA), silica membrane spin columns with collection tubes |
| Storage Conditions | Proteinase K stored at 2–8°C after reconstitution; all other components stored at room temperature (15–25°C); protect buffers from direct sunlight |
| Shelf Life | 18 months from date of manufacture; proteinase K solution stable for 12 months at 2–8°C after first use |
| Package Specifications | 50 preparations and 250 preparations; includes all required plasticware |
| Product Form | Liquid buffers and solutions; silica membrane spin columns; lyophilized proteinase K |
| Quality Control | Each lot tested for DNA yield and purity from whole human blood; absence of DNase/RNase verified by incubation with labeled substrates; qPCR performance validated using GAPDH and β-actin primer sets; endotoxin <0.1 EU/μg DNA |
| Key Features | No organic solvents or phenol extractions required; minimal hands-on time with spin column workflow; PCR-ready DNA eluted in TE or nuclease-free water; consistent yields across multiple sample types; compatible with automated liquid handling platforms |
For research use only, not for clinical use.
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