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| Product Name | Tandem Mass Tag (TMT) 10-Plex Isobaric Labeling Reagent Set |
| Catalog No. | PTR-HMM-0081 |
| Description | An isobaric chemical labeling reagent set enabling multiplexed quantitative proteomics analysis by tandem mass spectrometry. The ten-plex format allows simultaneous relative quantification of up to ten distinct peptide samples within a single mass spectrometry run. Each tag consists of an amine-reactive NHS ester group, a mass-normalizing spacer region, and a reporter ion that is released during higher-energy collisional dissociation. The reagents are fully compatible with common tryptic digestion and peptide cleanup workflows. |
| Intended Use | Multiplexed relative protein quantification across up to ten biological samples using isobaric tandem mass tag labeling and LC-MS/MS analysis. |
| Principle / Technology | Isobaric chemical labeling with reporter ion quantification |
| Detection Method | Tandem mass spectrometry |
| Sample Type | Peptide digests from proteins, cells, tissues, or biofluids |
| Performance Range / Specifications | Reporter ion mass range: 126–131 Da; labeling efficiency: ≥98% with optimized peptide-to-tag ratio |
| Sensitivity / LOD | Quantification dynamic range: 100-fold with CV ≤15% |
| Specificity | Selective for primary amine groups on peptide N-termini and lysine side chains |
| Reaction Conditions / Protocol | Labeling: 1 hr at room temperature in 100 mM TEAB buffer, pH 8.5; quench with hydroxylamine 15 min |
| Components / Formulation | Ten individual TMT reagent vials (0.8 mg each), hydroxylamine quenching solution, TEAB buffer, anhydrous acetonitrile |
| Storage Conditions | –20 °C, desiccated; avoid repeated freeze-thaw cycles |
| Shelf Life | 18 months from manufacture date |
| Package Specifications | 10-plex set (sufficient for 10 × 100 µg peptide labeling) |
| Product Form | Lyophilized reagents |
| Quality Control | Each lot verified by labeling a standard peptide mixture and confirming reporter ion ratios within ±15% of expected values |
| Key Features | Simultaneous ten-sample multiplexing reduces instrument time and inter-run variability |
| Purity | As specified per product type; recombinant proteins ≥90% by SDS-PAGE |
| Concentration | As specified on product label or certificate of analysis |
| Activity / Unit Definition | Binding capacity or enzymatic activity as specified per product documentation |
| Molecular Weight | Varies by product as specified in documentation |
| Source / Origin | Recombinant expression in E. coli, mammalian, or insect cell systems; synthetic chemistry for small molecule reagents |
| pH Range / Optimal pH | pH 7.0–8.0 for most protein applications |
| Shipping Conditions | Ambient for dry reagents; cold packs for liquid protein solutions; dry ice for frozen products |
| Expiration Date / Stability | 12–24 months at recommended storage temperature |
| Regulatory / Compliance | Research use; manufactured under ISO 9001 quality system |
| Compatibility | Compatible with standard SDS-PAGE, Western blot, mass spectrometry, and chromatography systems |
| Recommended Buffer System | PBS, Tris, or HEPES buffers as specified in product protocol |
| Application Notes / Precautions | Avoid repeated freeze-thaw cycles for protein solutions; use fresh aliquots; protect light-sensitive reagents |
| Batch-to-Batch Consistency | Protein concentration and activity within ±15% of reference lot |
For research use only, not for clinical use.
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