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| Product Name | Sulfo-Cyanine 5.5 NHS Ester (Water-Soluble Near-Infrared Cyanine Dye) |
| Catalog No. | RERE-0004 |
| Description | This sulfonated cyanine dye is built for work in water. Multiple sulfonate groups give high aqueous solubility, so labelling proceeds in buffer without an organic co-solvent and the dye stays well behaved in biological systems. The NHS ester form reacts with primary amines, and the 5.5 emission window near 694 nm makes the dye a standard pick for small-animal in vivo imaging as well as for tagging proteins, antibodies, peptides and nucleic acid molecules. |
| Intended Use | Amine-reactive near-infrared label for small-animal in vivo imaging and for tagging proteins, antibodies, peptides and nucleic acids. |
| Principle / Technology | Sulfonate substitution supplies water solubility; the NHS ester couples to primary amines, forming stable amide linkages that carry the near-infrared fluorophore onto the target molecule. |
| Detection Method | Fluorescence detection with excitation/emission of 675/694 nm. |
| Sample Type | Solid dye; no appearance value is stated on the page. |
| Performance Range / Specifications | CAS 2419286-92-1; molecular formula C44H42K3N3O16S4; molecular weight 1114.37 g/mol; Ex/Em 675/694 nm; high water solubility, no organic co-solvent needed. |
| Specificity | Near-infrared emission with low background autofluorescence; soluble in water and physiological buffers. |
| Reaction Conditions / Protocol | Dissolve in aqueous buffer or anhydrous DMSO as convenient, react with the amine-bearing substrate, then remove unreacted dye by purification. |
| Components / Formulation | One vial of dye; buffers and purification media are supplied by the user. |
| Storage Conditions | Store at -25 to -15 °C, protected from light. |
| Shelf Life | 12 months under the recommended storage conditions. |
| Package Specifications | 1 mg, 5 mg or 10 mg |
| Key Features | - Dissolves in water or buffer with no organic co-solvent - Near-infrared emission at 694 nm for low-background imaging - Suits small-animal in vivo imaging workflows - Labels proteins, antibodies, peptides and nucleic acids - One-year shelf life protected from light |
| Activity / Unit Definition | No unit definition; performance is defined by its spectra and amine coupling. |
| Molecular Weight | CAS 2419286-92-1; molecular formula C44H42K3N3O16S4; molecular weight 1114.37 g/mol. |
| Source / Origin | Synthetic sulfonated cyanine fluorophore, supplied as a purified solid. |
| pH Range / Optimal pH | Does not apply; pH is not a meaningful specification for this product type. |
| Expiration Date / Stability | Stable through the stated shelf life when kept protected from light at the recommended storage temperature. |
| Regulatory / Compliance | Restricted to research applications. |
| Compatibility | Compatible with standard fluorescence instrumentation - microscopes, plate readers, flow cytometers or imaging systems as appropriate to the format. |
| Recommended Buffer System | No buffer is included; the dye dissolves directly in aqueous buffers without an organic co-solvent. |
| Application Notes / Precautions | Handle protected from light, minimise freeze-thaw cycles by preparing aliquots, and verify that the detection channel matches the dye before use. |
| Batch-to-Batch Consistency | Each production lot is released against fixed acceptance criteria, keeping performance consistent between orders. |
| Model Type | Amine-reactive water-soluble near-infrared dye |
| Starting Material / Tissue Site | Synthesised sulfonated cyanine 5.5 fluorophore, esterified as the NHS ester |
| Delivery Standard | Pack sizes 1 mg, 5 mg and 10 mg |
| Turnaround Time | No turnaround figure is published; shipping is arranged when the order is confirmed. |
| Readouts / Downstream Assays | Excitation/emission 675/694 nm on a near-infrared channel. |
For research use only, not for clinical use.
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