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| Product Name | Soluble Starch Synthase (SSS) Activity Assay Kit, 50T/48S, 25T/24S |
| Catalog No. | SCAK-YJL-0199 |
| Detection Method | UV spectrophotometry, Spectrophotometer |
| Storage | Store at -20°C, 6 months |
| Intended Use | For research use only. |
| Note | For your safety and health, please wear lab coat, disposable gloves and mask during operation. |
Soluble Starch Synthase (SSS), classified under EC 2.4.1.21, is a key enzyme in the starch biosynthesis pathway of plants. It exists in a free state within starch bodies and plays a critical role in catalyzing the elongation of starch chains—specifically, it facilitates the transfer of glucose molecules from adenosine diphosphate glucose (ADPG) to starch primers (glucans). This reaction not only extends starch chains but also generates adenosine diphosphate (ADP) as a byproduct.The activity of SSS is closely linked to the synthesis of amylopectin, one of the two major components of starch (the other being amylose). Amylopectin contributes to the structural and functional properties of starch, such as its solubility, gelatinization temperature, and digestibility. For researchers in plant biology, agricultural science, and food science, measuring SSS activity is essential for understanding how plants regulate starch production.This SSS Activity Assay Kit is designed to quantify SSS activity accurately, supporting studies aimed at optimizing crop yields (by enhancing starch accumulation in edible parts of crops like grains and tubers), improving starch quality (for applications in food processing, biofuel production, and pharmaceutical excipients), and investigating the impact of environmental factors (e.g., temperature, drought, and nutrient availability) on plant starch metabolism. It also aids in genetic engineering research, where scientists seek to develop crop varieties with modified starch content or composition to meet industry-specific needs.
Utilizes UV spectrophotometry, a well-established and reliable detection method, ensuring accurate and reproducible quantification of SSS activity.
Available in two practical sizes: 50T/48S and 25T/24S, allowing researchers to choose the appropriate volume based on their experimental scale (e.g., small-scale preliminary tests or large-scale batch analyses).
Offers a clear and straightforward operation process, reducing the complexity of experimental setup and minimizing the risk of human error during sample handling and reagent preparation.
Includes necessary reagents for the assay (consistent with industry-standard kit configurations for enzyme activity detection), eliminating the need for researchers to source and validate individual components separately.
Stores at -20°C with a 6-month shelf life from receipt, providing long-term usability and reducing the frequency of kit restocking for labs with ongoing research needs.
Ensures high specificity for SSS activity: The assay principle targets the unique catalytic reaction of SSS (ADPG-dependent starch chain elongation), minimizing interference from other enzymes in plant tissue extracts.
Supports the analysis of plant tissue samples, which aligns with the primary research focus of most users (e.g., studying starch synthesis in crops like wheat, rice, corn, and potatoes).
Enables accurate result interpretation: The detection of SSS activity is based on measurable changes in UV absorbance, which can be easily quantified using a standard spectrophotometer—no specialized or high-cost equipment is required.
Prioritizes user safety: Clear operation notes (e.g., wearing lab coats, disposable gloves, and masks) are provided to minimize risks during sample and reagent handling.
Ensures sample flexibility: While fresh samples are recommended for optimal results, the kit’s compatible sample storage guidelines (consistent with industry practices for enzyme activity assays) allow for short-term storage of prepared samples if immediate testing is not possible, reducing experimental time pressure.
For research use only, not for clinical use.
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