- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | SNP Genotyping Primer Synthesis Service, Allele-Specific (AS-PCR Primers) |
| Catalog No. | PRIPRO-0029 |
| Description | Custom synthesis of allele-specific PCR primers for SNP genotyping by AS-PCR (Allele-Specific PCR) or ARMS-PCR (Amplification Refractory Mutation System). Two forward primers are designed with the 3′-terminal nucleotide matching either the wild-type or variant allele, paired with a common reverse primer. Supplied desalted with optional HPLC purification for enhanced allele discrimination. |
| Intended Use | SNP genotyping by allele-specific PCR; point mutation detection; pharmacogenomic testing; transgenic organism genotyping; genetic disease mutation screening; plant and animal molecular marker-assisted selection. |
| Principle / Technology | Allele-specific forward primers have the discriminating nucleotide at the 3′-terminus; Taq DNA polymerase extends only when the 3′ base pairs correctly with the template; a mismatch at the 3′ end prevents amplification, enabling allele discrimination on agarose gel or qPCR |
| Detection Method | Customer provides SNP sequence context with variant nucleotide position indicated; primers designed for optimal Tm 58-62 °C with 3′ mismatch discrimination; supplied as two forward allele-specific primers plus common reverse primer; desalted standard, HPLC optional |
| Sample Type | Customer provides FASTA sequence of SNP locus (≥100 bp flanking the variant); indicate wild-type and variant alleles |
| Components / Formulation | Two allele-specific forward primers + one common reverse primer (3 oligonucleotides total per SNP); synthesis scale 25-200 nmol; purification: desalted (standard), HPLC, or PAGE |
| Storage Conditions | Lyophilized: -20 °C; resuspended in TE buffer (pH 8.0): -20 °C; avoid repeated freeze-thaw |
| Shelf Life | Lyophilized: 24 months at -20 °C; in solution: 12 months at -20 °C |
| Package Specifications | Custom service; 25 nmol, 50 nmol, 100 nmol, 200 nmol synthesis scales; 3 oligonucleotides per SNP assay |
| Product Form | Lyophilized DNA oligonucleotides in screw-cap tubes; resuspension instructions included |
| Key Features | Custom allele-specific primer design included; desalted or HPLC purification; synthesis scale 25-200 nmol; quality control by MALDI-TOF mass spectrometry per oligonucleotide; typical delivery 3-5 business days; validated for AS-PCR and ARMS-PCR applications |
| Purity | Desalted: >85% full-length by HPLC; HPLC: >90% full-length; PAGE: >95% full-length; each oligonucleotide verified by MALDI-TOF MS |
| Concentration | As specified in synthesis report; typically lyophilized or at 100 µM in TE |
| Activity / Unit Definition | Primer efficiency 90–110% in qPCR; Tm within ±2 °C of calculated value |
| Molecular Weight | As specified per MALDI-TOF certificate; within 0.1% of theoretical mass |
| Source / Origin | Solid-phase phosphoramidite chemical synthesis |
| pH Range / Optimal pH | Stable in TE buffer pH 7.0–8.0; avoid acidic conditions causing depurination |
| Shipping Conditions | Ambient temperature; lyophilized oligonucleotides stable at RT for 2 weeks during transit; long-term storage at -20 °C upon receipt |
| Expiration Date / Stability | 24 months lyophilized at -20 °C; 12 months in TE buffer at -20 °C; limit freeze-thaw to <10 cycles |
| Regulatory / Compliance | For laboratory and research use only; RUO; ISO 9001; standard oligonucleotide synthesis — no special regulatory requirements |
| Compatibility | Compatible with standard PCR, qPCR, and sequencing platforms |
| Recommended Buffer System | Resuspend in nuclease-free water or TE buffer pH 8.0 |
| Application Notes / Precautions | Protect fluorescent probes from light; avoid repeated freeze-thaw cycles; aliquot for single use |
| Batch-to-Batch Consistency | MALDI-TOF mass confirmation per lot; qPCR performance within 0.5 Ct of reference lot |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
