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| Product Name | Custom DNA Oligonucleotide Synthesis Service (100 nmol, PAGE Purified) |
| Catalog No. | PRIPRO-0003 |
| Description | A high-scale custom DNA oligonucleotide synthesis service delivering 100 nanomole yield with polyacrylamide gel electrophoresis (PAGE) purification. PAGE purification achieves the highest resolution separation of full-length oligonucleotides from truncated synthesis by-products, including single-base (n–1) deletions. This purification method is the gold standard for long oligonucleotides (>40 bases) and applications demanding single-nucleotide resolution. Each oligo is gel-eluted, desalted, and lyophilized. Quality is verified by analytical PAGE and mass spectrometry. Recommended for demanding applications including gene synthesis, site-directed mutagenesis with long primers, and electrophoretic mobility shift assays (EMSA). |
| Intended Use | Optimized for gene synthesis assembly, long-primer site-directed mutagenesis, EMSA probe preparation, antisense oligonucleotide research, and any application where n–1 contamination must be minimized. |
| Principle / Technology | Solid-phase phosphoramidite synthesis followed by denaturing PAGE separation based on length; full-length band excised and eluted |
| Detection Method | Analytical PAGE and MALDI-TOF mass spectrometry |
| Sample Type | Template DNA, plasmid DNA, or genomic DNA for downstream applications |
| Performance Range / Specifications | 5–120 bases; optimal purification for 30–100 nt range |
| Sensitivity / LOD | N/A — custom synthesis reagent |
| Specificity | PAGE purification removes n–1 and shorter species; ≥97% full-length product by analytical PAGE; single-base resolution achieved |
| Reaction Conditions / Protocol | Resuspend in nuclease-free water or TE buffer. Prepare 100 µM stock. For gene synthesis, use equimolar amounts of overlapping oligos at 1–10 µM each in assembly reaction. |
| Components / Formulation | Lyophilized PAGE-purified oligonucleotide in screw-cap tube; COA enclosed |
| Storage Conditions | –20 °C in the dark; desiccated storage recommended for long-term stability |
| Shelf Life | 24 months dry at –20 °C; 6 months in solution at –20 °C (aliquoted) |
| Package Specifications | Screw-cap microcentrifuge tube with 100 nmol nominal synthesis scale |
| Product Form | Lyophilized white to slightly translucent pellet |
| Quality Control | Analytical PAGE confirming ≥97% full-length purity; MALDI-TOF MS mass confirmation; A260 quantification; residual acrylamide tested below detection limit. |
| Key Features | PAGE purification ensures single-base resolution; ideal for long oligos >40 nt; minimal n–1 contamination; mass spectrometry confirmation; suitable for demanding gene synthesis applications. |
| Purity | ≥97% by analytical PAGE; single-band product |
| Concentration | Lyophilized from 100 nmol synthesis scale; typical yield 30–70 OD260 units |
| Activity / Unit Definition | High-purity full-length oligo suitable for high-efficiency cloning and gene assembly reactions |
| Molecular Weight | Calculated per sequence composition; provided on COA |
| Source / Origin | Synthetic phosphoramidite chemistry; no biological source materials |
| pH Range / Optimal pH | Stable in pH 6.0–8.5; resuspension recommended at pH 7.5–8.0 |
| Shipping Conditions | Ambient temperature as dry pellet; cold pack optional |
| Expiration Date / Stability | 24 months at –20 °C dry; 12 months at –20 °C in TE; avoid freeze-thaw cycles |
| Regulatory / Compliance | Manufactured under ISO 9001 quality management system; for research use only; not for diagnostic applications |
| Compatibility | Compatible with all major DNA polymerases (Taq, Pfu, Phusion, Q5, KOD); suitable for Gibson assembly, Golden Gate, and traditional ligation cloning |
| Recommended Buffer System | Nuclease-free water or TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0) |
| Application Notes / Precautions | For long oligos, verify full-length purity by analytical PAGE before use in gene assembly. Store aliquots to avoid repeated freeze-thaw. Centrifuge briefly before opening to collect pellet. |
| Batch-to-Batch Consistency | PAGE purity maintained within ±2% across synthesis batches; MS mass accuracy within ±0.05% |
For research use only, not for clinical use.
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