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| Product Name | Custom DNA Oligonucleotide Synthesis Service (1 µmol, Large-Scale) |
| Catalog No. | PRIPRO-0004 |
| Description | A large-scale custom DNA oligonucleotide synthesis at the 1 micromole scale, designed for high-volume research needs and pilot-scale production. Synthesized by solid-phase phosphoramidite chemistry with HPLC or PAGE purification as selected. The large synthesis scale yields substantial amounts of full-length oligonucleotide suitable for NMR structural studies, X-ray crystallography, antisense oligonucleotide screening, aptamer selection (SELEX), and diagnostic assay development. Each batch is rigorously tested by HPLC, mass spectrometry, and endotoxin assay. Custom formulations including salt forms and concentration adjustments are available. Supplied as a dried pellet with detailed analytical documentation. |
| Intended Use | Designed for NMR structural biology, X-ray crystallography, SELEX aptamer selection, antisense oligonucleotide functional studies, diagnostic assay development, and any application requiring milligram quantities of defined-sequence oligonucleotide. |
| Principle / Technology | Solid-phase phosphoramidite synthesis at 1 µmol scale on CPG support; optional HPLC or PAGE purification based on length and purity requirements |
| Detection Method | HPLC quantification, MALDI-TOF MS, endotoxin LAL assay |
| Sample Type | Various — platform reagent for downstream structural or functional studies |
| Performance Range / Specifications | 2–60 bases standard; longer sequences available by custom quotation |
| Sensitivity / LOD | N/A — custom large-scale synthesis |
| Specificity | Purification method selected per customer requirements; ≥90% full-length for HPLC, ≥97% for PAGE |
| Reaction Conditions / Protocol | Dissolve in appropriate buffer based on downstream application. For NMR, resuspend in D2O or desired buffer system. For SELEX, prepare 100 µM to 1 mM stock solutions. |
| Components / Formulation | One vial containing lyophilized large-scale oligonucleotide; certificate of analysis with HPLC trace and MS spectrum |
| Storage Conditions | –20 °C or –80 °C desiccated; protect from light and moisture |
| Shelf Life | 24 months dry at –20 °C; 12 months in solution at –20 °C |
| Package Specifications | Glass vial or multiple screw-cap tubes with total 1 µmol nominal yield (actual yield sequence-dependent, typically 0.5–0.8 µmol) |
| Product Form | Lyophilized white powder or pellet; custom salt forms available |
| Quality Control | HPLC purity analysis; MALDI-TOF or ESI-MS mass confirmation; endotoxin testing (<0.1 EU/µg); A260 quantification; residual solvent analysis; heavy metal testing. |
| Key Features | Milligram-scale yield for structural biology and screening; choice of purification method; custom salt form and buffer options; endotoxin testing included; detailed analytical package; batch reservation available for long-term projects. |
| Purity | ≥90% HPLC or ≥97% PAGE, depending on purification selection |
| Concentration | Lyophilized from 1 µmol synthesis scale; typical yield 0.5–0.8 µmol (300–500 OD260 units, sequence dependent) |
| Activity / Unit Definition | Sequence-specific hybridization confirmed; functional in enzymatic reactions including phosphorylation, ligation, and polymerization |
| Molecular Weight | Calculated from sequence; ESI-MS accurate mass reported on COA |
| Source / Origin | Synthetic; phosphoramidite monomers from certified suppliers |
| pH Range / Optimal pH | Customizable buffer formulation; native oligo stable pH 6.0–8.5 |
| Shipping Conditions | Ambient temperature as lyophilized powder; express courier available |
| Expiration Date / Stability | 24 months desiccated at –20 °C; 12 months at –20 °C in sterile nuclease-free water or TE buffer |
| Regulatory / Compliance | ISO 9001:2015 certified production; full traceability of raw materials; for research use only; custom GMP documentation available upon request |
| Compatibility | Compatible with standard molecular biology workflows; suitable for NMR (D2O exchange), crystallography, and SELEX protocols |
| Recommended Buffer System | Custom buffer formulation available; standard: nuclease-free water or TE (pH 8.0) |
| Application Notes / Precautions | For NMR applications, request ammonium acetate salt form for improved solubility. Aliquot upon resuspension to avoid repeated freeze-thaw. Lyophilized aliquots can be prepared for long-term storage. |
| Batch-to-Batch Consistency | HPLC retention time and MS mass accuracy maintained within ±2% and ±0.05% respectively across production lots |
For research use only, not for clinical use.
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