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| Product Name | Slide-A-Lyzer Dialysis Cassette (10K MWCO) |
| Catalog No. | PTR-HMM-0072 |
| Description | Single-use dialysis cassettes with a regenerated cellulose membrane and 10,000 Dalton molecular weight cut-off for efficient buffer exchange, desalting, and removal of small-molecule contaminants from protein samples. |
| Intended Use | Buffer exchange, desalting, and removal of low-molecular-weight contaminants from protein solutions in volumes ranging from 0.5 to 3 mL per cassette. |
| Principle / Technology | Size-selective diffusion across a regenerated cellulose membrane with a defined pore size: molecules smaller than 10 kDa pass through the membrane into the surrounding buffer, while proteins larger than 10 kDa are retained within the cassette. |
| Detection Method | Passive dialysis against a large-volume reservoir of the desired buffer; no pump or pressure required |
| Sample Type | Protein solutions, antibody preparations, peptide mixtures, nucleic acid-protein complexes; compatible with common biological buffers |
| Performance Range / Specifications | Sample volume range: 0.5–3 mL per cassette; >95% buffer exchange achieved with three buffer changes of 200× sample volume each |
| Sensitivity / LOD | Recovery of proteins at concentrations as low as 10 μg/mL with proper handling |
| Specificity | Retention of proteins and macromolecules larger than 10,000 Daltons; low-molecular-weight buffer salts, preservatives, and small-molecule reagents are removed |
| Reaction Conditions / Protocol | Hydrate cassette in buffer for 2 minutes, inject sample through the port using a syringe, remove air, float cassette in 200–500× volume of target buffer with stirring, dialyze for 2–4 hours at 4°C with three buffer changes, withdraw sample using a fresh syringe |
| Components / Formulation | 10 dialysis cassettes individually packaged, disposable syringes with 21-gauge needles for sample injection and withdrawal, flotation buoys |
| Storage Conditions | Room temperature in sealed packaging; avoid exposure to high humidity |
| Shelf Life | 24 months |
| Package Specifications | 10 cassettes per package |
| Product Form | Single-use dialysis cassette with regenerated cellulose membrane |
| Quality Control | Each lot tested for molecular weight cut-off accuracy using characterized protein standards; membrane integrity verified; endotoxin levels tested (<0.1 EU per cassette) |
| Key Features | Single-use format eliminates cleaning and cross-contamination concerns; the flotation buoy design ensures the cassette remains at the buffer-air interface for maximum surface area exposure; high recovery rates minimize sample loss |
For research use only, not for clinical use.
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