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| Product Name | siRNA Duplex Synthesis Service, Custom Sequence, HPLC-Purified |
| Catalog No. | PRIPRO-0033 |
| Description | Custom synthesis of siRNA duplexes (21-23 nt) with symmetric or asymmetric 3′ overhangs (dTdT or UU) for RNA interference (RNAi) gene knockdown studies. Each siRNA duplex is HPLC-purified to >90% purity, annealed, and supplied as a ready-to-transfect solution or lyophilized. Includes a negative control (scrambled sequence) siRNA for experimental controls. |
| Intended Use | Specific gene knockdown in mammalian cells; RNAi pathway research; functional genomics screening; target validation; siRNA therapeutic development; in vivo gene silencing studies. |
| Principle / Technology | Chemically synthesized 21-23 nt RNA oligonucleotides with complementary sense and antisense strands; annealed to form siRNA duplex with 3′ dTdT overhangs recognized by Dicer and RISC; antisense strand guides RISC to complementary mRNA for cleavage and degradation |
| Detection Method | Customer provides target gene mRNA sequence (RefSeq accession or FASTA); optimal siRNA sequences designed using proprietary algorithm considering specificity, thermodynamic asymmetry, and off-target potential; 3-4 siRNA sequences recommended per target for initial screening |
| Sample Type | Customer provides gene name, RefSeq mRNA accession, or target mRNA sequence (≥100 bp); specify species (human, mouse, rat, etc.) |
| Components / Formulation | siRNA duplex (sense + antisense strands, annealed), negative control siRNA (scrambled, non-targeting), siRNA resuspension buffer (nuclease-free, pH 7.4); 5-20 nmol per duplex standard scale |
| Storage Conditions | Lyophilized: 4 °C or -20 °C; resuspended: -20 °C in aliquots; avoid repeated freeze-thaw; protect from RNase contamination |
| Shelf Life | Lyophilized: 24 months at -20 °C; resuspended: 6 months at -20 °C (aliquoted), 2 weeks at 4 °C |
| Package Specifications | 5 nmol, 10 nmol, 20 nmol, 50 nmol, 100 nmol per duplex; negative control included |
| Product Form | Lyophilized siRNA duplex (annealed) or ready-to-use solution (100 µM in resuspension buffer) |
| Key Features | HPLC-purified >90% full-length duplex; 3′ dTdT overhangs for enhanced stability; proprietary design algorithm minimizes off-targets; negative control siRNA included; endotoxin-tested (<0.1 EU/nmol) for in vivo applications; chemically modified options: 2′-O-methyl, 2′-F, phosphorothioate; typical delivery 5-7 business days |
| Purity | HPLC-purified >90% duplex; individual strands >95% by HPLC; endotoxin <0.1 EU/nmol; duplex confirmed by non-denaturing PAGE |
| Concentration | As specified in synthesis report; typically lyophilized or at 100 µM in TE |
| Activity / Unit Definition | Primer efficiency 90–110% in qPCR; Tm within ±2 °C of calculated value |
| Molecular Weight | As specified per MALDI-TOF certificate; within 0.1% of theoretical mass |
| Source / Origin | Solid-phase phosphoramidite chemical synthesis |
| pH Range / Optimal pH | Stable in TE buffer pH 7.0–8.0; avoid acidic conditions causing depurination |
| Shipping Conditions | Ambient; lyophilized siRNA stable at RT 2 weeks; resuspended siRNA ships on cold pack; protect from RNase |
| Expiration Date / Stability | 24 months lyophilized at -20 °C; 6 months resuspended at -20 °C aliquoted; use RNase-free technique for resuspension; discard if non-denaturing PAGE shows single-strand dissociation |
| Regulatory / Compliance | For laboratory and research use only; RUO; siRNAs are synthetic — no biohazard; for therapeutic use, GMP-grade synthesis available upon request; ISO 9001 |
| Compatibility | Compatible with standard PCR, qPCR, and sequencing platforms |
| Recommended Buffer System | Resuspend in nuclease-free water or TE buffer pH 8.0 |
| Application Notes / Precautions | Protect fluorescent probes from light; avoid repeated freeze-thaw cycles; aliquot for single use |
| Batch-to-Batch Consistency | MALDI-TOF mass confirmation per lot; qPCR performance within 0.5 Ct of reference lot |
For research use only, not for clinical use.
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