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| Product Name | Single-Component TMB Substrate Solution for HRP-Based Western Blotting |
| Catalog No. | RERE-0064 |
| Description | One-component 3,3',5,5'-tetramethylbenzidine substrate with dedicated stabilisers and no organic solvent. Horseradish peroxidase converts TMB to a blue intermediate absorbing at 370 nm and 652 nm, and after acid termination the yellow product peaks at 450 nm. The precipitate-insoluble formulation deposits a deep purple product directly on the membrane, giving permanent staining for Western blot as well as Northern and Southern blot detection with HRP-labelled probes. |
| Intended Use | Membrane visualisation of target proteins in Western blot and of target nucleic acids in Northern and Southern blot with HRP labels. |
| Detection Method | HRP-labelled probes or antibodies on membranes |
| Performance Range / Specifications | Sensitivity at least equal to two-component AB systems |
| Storage Conditions | 2-8 °C protected from light |
| Shelf Life | 1 year |
| Package Specifications | 100 mL |
| Product Form | Single-component liquid substrate, precipitate-insoluble type |
| Recommended Buffer System | TMB with stabilisers in a DMF-free and DMSO-free single solution |
For research use only, not for clinical use.
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