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| Product Name | Single-Component TMB Substrate Solution for HRP-Based ELISA |
| Catalog No. | RERE-0063 |
| Description | One-component ready-to-use 3,3',5,5'-tetramethylbenzidine substrate containing TMB, hydrogen peroxide and dedicated stabilisers in a single solution free of DMF and DMSO. Under horseradish peroxidase catalysis TMB is oxidised to a blue product absorbing at 370 nm and 652 nm; after stopping with 2 M sulfuric acid the yellow end product is read at 450 nm. The precipitate-soluble formulation resists settling and any crystals redissolve readily, avoiding the mixing errors of conventional two-bottle AB systems. |
| Intended Use | Colorimetric ELISA, solution-phase HRP detection and peroxidase activity measurement in blood or haemoglobin samples. |
| Detection Method | Horseradish peroxidase (HRP) activity via TMB oxidation |
| Performance Range / Specifications | Sufficient for approximately 500 microplate wells per 100 mL; sensitivity at least equal to two-component AB systems and superior to ABTS or OPD |
| Reaction Conditions / Protocol | Add 200 µL of substrate per well, incubate, stop with 2 M sulfuric acid and read absorbance at 450 nm. |
| Storage Conditions | 4 °C protected from light |
| Shelf Life | 1 year |
| Package Specifications | 100 mL |
| Product Form | Single-component liquid substrate |
| Shipping Conditions | Cold pack |
| Recommended Buffer System | TMB, hydrogen peroxide and stabilisers in a DMF-free and DMSO-free aqueous system |
For research use only, not for clinical use.
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