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| Product Name | Single-Component BCIP/NBT Substrate Solution for AP-Based Western Blotting |
| Catalog No. | RERE-0067 |
| Description | One-component combination of 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium, among the best substrate pairs for alkaline phosphatase. AP hydrolyses BCIP to a reactive intermediate that reduces NBT, depositing an insoluble deep blue to blue-purple precipitate at the detection site. All reagents arrive pre-mixed with dedicated stabilisers, removing the two-bottle mixing and extra AP buffer that conventional systems require. |
| Intended Use | Membrane detection of target proteins in Western blot with AP-labelled antibodies. |
| Detection Method | Alkaline phosphatase (AP) activity via BCIP/NBT deposition |
| Storage Conditions | 2-8 °C protected from light |
| Shelf Life | 1 year |
| Package Specifications | 100 mL |
| Product Form | Single-component liquid substrate |
| Recommended Buffer System | BCIP and NBT with dedicated stabilisers in one ready-to-use solution |
For research use only, not for clinical use.
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