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| Product Name | Serum/Plasma Circulating Free DNA Extraction Kit (Magnetic Bead-Based, High Sensitivity) |
| Catalog No. | DREK-0024 |
| Description | A magnetic bead-based kit optimized for the extraction of circulating cell-free DNA from human serum and plasma. The kit employs optimized binding chemistry for the efficient capture of short DNA fragments as small as 50 base pairs, which are characteristic of circulating tumor DNA and fetal DNA. The magnetic bead format enables automated processing on liquid handling platforms. Extracted cfDNA is suitable for digital PCR, next-generation sequencing, and qPCR-based liquid biopsy assays. |
| Intended Use | High-recovery purification of circulating cell-free DNA from plasma and serum for liquid biopsy applications. |
| Principle / Technology | Magnetic bead-based nucleic acid binding optimized for short DNA fragment capture |
| Detection Method | Fluorometric quantification (Qubit); Bioanalyzer or TapeStation for fragment size distribution; qPCR or ddPCR for specific target recovery |
| Sample Type | Human plasma (EDTA preferred) or serum; fresh or frozen specimens |
| Performance Range / Specifications | DNA recovery: ≥85% for 170 bp fragment; fragment capture range: 50–1000 bp; input volume: 0.2–4 mL |
| Sensitivity / LOD | Consistent recovery from plasma samples containing ≤1 ng/mL cfDNA |
| Specificity | Non-selective DNA binding; captures both genomic and mitochondrial DNA fragments; no sequence bias as verified by NGS |
| Reaction Conditions / Protocol | Digest plasma with proteinase K; bind cfDNA to magnetic beads in binding buffer; wash 2×; elute in 30–50 µL low-TE buffer |
| Components / Formulation | Proteinase K, binding buffer, magnetic bead suspension, wash buffer 1, wash buffer 2, elution buffer (low-TE), carrier RNA |
| Storage Conditions | Proteinase K and carrier RNA: –20 °C; other reagents: room temperature |
| Shelf Life | 12 months |
| Package Specifications | 50 preparations |
| Product Form | Liquid buffers; magnetic bead suspension |
| Quality Control | cfDNA recovery validated by spiking fragmented DNA standard into cfDNA-depleted plasma; fragment size distribution confirmed by Bioanalyzer |
| Key Features | Optimized for short DNA fragments ensures high recovery of tumor-derived ctDNA typically degraded to 130–170 bp |
| Purity | Extracted nucleic acid A260/A280: 1.8–2.0 (DNA), 1.9–2.1 (RNA); A260/A230 ≥1.8 |
| Concentration | Elution volume and yield as specified per sample type |
| Activity / Unit Definition | DNA/RNA binding capacity per column or per mg beads as specified |
| Molecular Weight | Genomic DNA >50 kb; RNA 0.1–10 kb range as applicable |
| Source / Origin | Silica membrane or magnetic bead technology; recombinant Proteinase K |
| pH Range / Optimal pH | Binding buffer pH 5.0–7.0 for silica-based binding |
| Shipping Conditions | Ambient temperature for most components; Proteinase K shipped with cold packs |
| Expiration Date / Stability | 12 months at recommended storage temperature |
| Regulatory / Compliance | Research use; IVD-grade versions available with full documentation |
| Compatibility | Compatible with manual spin columns, vacuum manifolds, and automated liquid handlers |
| Recommended Buffer System | Chaotropic salt binding buffer; ethanol-based wash; low-salt TE or water elution |
| Application Notes / Precautions | Pre-warm elution buffer to improve yield; avoid cross-contamination between samples; change pipette tips between steps |
| Batch-to-Batch Consistency | DNA yield and purity within ±15% of reference lot on standardized blood sample |
For research use only, not for clinical use.
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