- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Magnetic Bead-Based Genomic DNA Extraction Kit (Whole Blood) |
| Catalog No. | DREK-0002 |
| Description | A magnetic bead-based genomic DNA purification kit designed for high-yield, high-purity extraction of genomic DNA from whole blood samples. The optimized lysis chemistry ensures complete digestion of blood components including hemoglobin and leukocytes, while the magnetic silica bead system achieves consistent DNA binding and elution with minimal fragmentation. The extracted genomic DNA has an average fragment size above 30 kb and performs reliably in long-range PCR, qPCR, and whole-genome sequencing applications. |
| Intended Use | Isolation of high-molecular-weight genomic DNA from human whole blood samples (fresh or frozen, anticoagulated with EDTA, citrate, or heparin) for molecular biology applications including PCR, qPCR, Southern blotting, genotyping, Sanger sequencing, and next-generation sequencing library preparation. |
| Principle / Technology | Whole blood is lysed in a guanidine-based buffer containing proteinase K, which digests proteins and releases genomic DNA from nucleated cells. The DNA binds selectively to silica-coated magnetic beads under high-salt chaotropic conditions. After magnetic capture and serial wash steps to remove heme, proteins, and salts, purified genomic DNA is eluted in Tris-EDTA buffer or nuclease-free water. |
| Detection Method | UV spectrophotometry (A260/A280 and A260/A230); agarose gel electrophoresis with high-molecular-weight markers; fluorometric quantitation with PicoGreen or Qubit dsDNA HS assay; PCR amplification of GAPDH, β-globin, and mitochondrial targets for quality assessment |
| Sample Type | Human whole blood (EDTA, citrate, or heparin anticoagulated) fresh or frozen at -20°C or -80°C; compatible with blood stored at 2-8°C for up to 7 days |
| Performance Range / Specifications | Input volume: 100-500 µL whole blood; yield from 200 µL normal human blood: 4-10 µg genomic DNA; A260/A280: 1.75-1.90; A260/A230: >1.8; average fragment size: 30-50 kb with gentle handling; DNA suitable for amplification of targets up to 20 kb |
| Sensitivity / LOD | Recovers genomic DNA from as few as 10 µL fingerstick blood; minimum detectable yield by PicoGreen: 0.5 ng; reliably recovers DNA from partially degraded blood samples stored for extended periods |
| Specificity | Highly specific for double-stranded genomic DNA; no co-purification of RNA detectable by agarose gel; heme contamination below A410 threshold; no detectable PCR inhibitors in standard qPCR assays; minimal mitochondrial DNA enrichment (consistent with genomic representation) |
| Reaction Conditions / Protocol | Transfer 100-500 µL whole blood to microcentrifuge tube; add lysis/binding buffer and proteinase K; incubate at 56°C for 10-20 minutes with occasional vortexing; add isopropanol and magnetic bead suspension; incubate 5 minutes at room temperature with mixing; separate beads magnetically; wash with wash buffer 1, then wash buffer 2 twice; air-dry beads 2-3 minutes; elute in 50-200 µL Tris-EDTA buffer at 56°C for 5-10 minutes; total protocol: 35-50 minutes for 1-24 samples |
| Components / Formulation | Lysis/binding buffer (guanidine hydrochloride, Tris-HCl pH 8.0, EDTA, SDS), proteinase K solution (20 mg/mL in storage buffer), magnetic silica bead suspension (50 mg/mL in storage buffer), wash buffer 1 concentrate (guanidine hydrochloride, Tris-HCl, NaCl, ethanol), wash buffer 2 concentrate (Tris-HCl, NaCl, EDTA, ethanol), elution buffer (10 mM Tris-HCl pH 8.5, 0.5 mM EDTA), magnetic separation rack |
| Storage Conditions | Buffer components at room temperature (15-25°C); proteinase K at 2-8°C; magnetic bead suspension at 2-8°C; protect buffers from direct sunlight; avoid freezing magnetic beads |
| Shelf Life | 24 months from date of manufacture; proteinase K solution stable for 12 months at 2-8°C after first use |
| Package Specifications | 50 preparations (200 µL blood per prep), 200 preparations; all buffers and consumables included; magnetic rack sold separately |
| Product Form | Liquid buffers and solutions; silica-coated magnetic bead suspension; proteinase K in glycerol-based storage buffer |
| Quality Control | Each lot validated with human whole blood: yield 4-10 µg from 200 µL input; A260/A280: 1.75-1.90; fragment size >30 kb by agarose gel; PCR amplification of 5 kb and 10 kb targets confirmed; GAPDH qPCR Ct shift <0.5 vs reference; endotoxin <0.1 EU/µg DNA; no detectable DNase or RNase activity |
| Key Features | Consistent yields across variable whole blood samples including anemic and leukopenic specimens; minimal fragmentation with gentle vortex mixing; high molecular weight DNA suitable for long-read sequencing; minimal heme contamination; magnetic bead format compatible with automation; rapid protocol under 50 minutes |
| Purity | A260/A280: 1.75-1.90; A260/A230: >1.8; heme absorbance at 410 nm <0.02 |
| Concentration | Typical DNA concentration 40-100 ng/µL in 50-100 µL elution volume from 200 µL normal whole blood |
| Activity / Unit Definition | Not applicable for extraction kits |
| Molecular Weight | Not applicable; genomic DNA is heterogeneous high-molecular-weight polymer |
| Source / Origin | Silica-coated magnetic beads are synthetic; proteinase K is recombinantly expressed from Tritirachium album in a fungal expression system; all buffers are chemically synthesized |
| pH Range / Optimal pH | Lysis buffer working pH 7.8-8.2; wash buffer 1 pH 6.0-6.5; wash buffer 2 pH 7.0-7.5; elution buffer pH 8.3-8.7 |
| Shipping Conditions | Room temperature shipping for all components; cold packs recommended for proteinase K during summer months or tropical shipping |
| Expiration Date / Stability | 24 months shelf life unopened; accelerated aging at 45°C for 21 days confirms stability equivalent to 24 months; proteinase K retains >90% activity after 12 months at 2-8°C post-opening |
| Regulatory / Compliance | For research use only; not for use in diagnostic procedures; manufactured in compliance with ISO 9001 quality management system |
| Compatibility | Eluted genomic DNA compatible with all major PCR master mixes, qPCR systems, Sanger sequencing chemistries, restriction enzyme digestion, and NGS library preparation kits; compatible with KingFisher, Maxwell, and other magnetic particle processing platforms |
| Recommended Buffer System | Guanidine hydrochloride and SDS-based lysis; Tris-HCl buffered wash solutions; Tris-EDTA elution buffer optimized for long-term DNA stability |
| Application Notes / Precautions | For blood samples with high leukocyte counts, reduce input volume proportionally; avoid vigorous vortexing of eluted DNA to preserve high molecular weight; pre-warm elution buffer to 56°C for optimal yield; frozen blood should be thawed quickly at 37°C and processed immediately; do not freeze magnetic beads |
| Batch-to-Batch Consistency | Between-lot DNA yield CV <12% measured across 10 independent blood donors; A260/A280 variation between lots <0.05; magnetic bead binding capacity controlled to ±10% of specification per lot; buffer conductivity and pH verified for each batch |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
