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| Product Name | RNase-Free DNase I |
| Catalog No. | IEC-HMM-0118 |
| Description | A high-activity deoxyribonuclease for digesting single- and double-stranded DNA while maintaining low RNase contamination. The enzyme cleaves phosphodiester bonds to generate products with 5-prime phosphate and 3-prime hydroxyl termini. |
| Intended Use | DNA removal during RNA isolation, in vitro transcription and RT-PCR workflows; DNase I footprinting; nick translation; random-fragment DNA library preparation. |
| Principle / Technology | Endonucleolytic cleavage of phosphodiester bonds in single- and double-stranded DNA. |
| Detection Method | Enzymatic DNA digestion. |
| Sample Type | DNA-containing RNA preparations, in vitro transcription mixtures and purified DNA substrates. |
| Performance Range / Specifications | Available as 1,000 U and 10,000 U; in the reported activity test, 0.01 U completely digested 200 ng plasmid DNA under the specified assay conditions. |
| Specificity | Digests single- and double-stranded DNA; low detectable RNase contamination. |
| Reaction Conditions / Protocol | Add the required enzyme amount to the DNA-containing sample and incubate under the buffer and temperature conditions specified for the application. |
| Storage Conditions | Store at -30 to -15°C. |
| Package Specifications | 1,000 U; 10,000 U. |
| Product Form | RNase-free deoxyribonuclease enzyme. |
| Quality Control | RNase carryover was evaluated using an RNase-detection assay and was not detected in the reported test. |
| Key Features | High DNase activity; low RNase carryover; good storage stability; broad DNA-removal applications. |
| Activity / Unit Definition | Enzyme activity is supplied in units; the source page lists 1,000 U and 10,000 U package formats. |
| Shipping Conditions | Ship at 0°C or below. |
| Compatibility | RNA purification, in vitro transcription, RT-PCR, footprinting, nick translation and DNA-fragmentation workflows. |
| Application Notes / Precautions | Use RNase-free consumables for RNA applications and inactivate or remove DNase I before downstream enzymatic reactions when required. |
For research use only, not for clinical use.
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