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| Product Name | RNA 6000 Nano Analysis Kit for Bioanalyzer |
| Catalog No. | NATR-HMM-0135 |
| Description | A microfluidic chip-based reagent kit for the automated electrophoretic separation and analysis of total RNA and mRNA samples on a benchtop capillary electrophoresis platform. The kit provides RNA concentration, size distribution, and an RNA Integrity Number (RIN) for objective quality assessment. |
| Intended Use | Quality control assessment of total RNA samples prior to gene expression analysis, microarray hybridization, RT-qPCR, and RNA sequencing. Determination of RNA concentration, purity, and integrity using the RNA Integrity Number (RIN) as a standardized quality metric. |
| Principle / Technology | RNA samples are electrophoretically separated through a micro-fabricated capillary channel filled with a sieving polymer matrix and a fluorescent intercalating dye. As RNA molecules migrate through the microchannels under an applied electric field, the dye intercalates and fluoresces, enabling laser-induced fluorescence detection. The software algorithm deconvolutes the electropherogram and assigns a RIN value from 1 (completely degraded) to 10 (intact) based on the ratio of ribosomal bands and the presence of degradation products. |
| Detection Method | Laser-induced fluorescence detection using a dedicated benchtop capillary electrophoresis instrument; automated data analysis with software providing RNA concentration, rRNA ratio (28S/18S or 23S/16S for eukaryotes and prokaryotes respectively), RIN score, and virtual gel image |
| Sample Type | Total RNA from eukaryotic cells and tissues; total RNA from prokaryotic cells; purified mRNA (if within the concentration range); in vitro transcribed RNA; RNA from FFPE and laser-capture microdissection samples |
| Performance Range / Specifications | Quantitative range: 25–500 ng/μL total RNA (15–300 ng/μL for eukaryotic); qualitative range: 5–500 ng/μL; minimum sample volume: 1 μL; maximum RNA size: 6,000 nucleotides; analysis time: ~30 minutes per chip (11–12 samples plus ladder) |
| Sensitivity / LOD | Reliably detects 5 ng/μL total RNA with degradation products; RIN precision: ±0.3 across technical replicates; can distinguish 28S/18S ratios from 0.5 to 3.0 |
| Specificity | Species-independent analysis with appropriate reference ladder; distinguishes 18S and 28S ribosomal peaks for eukaryotes, 16S and 23S for prokaryotes; no cross-reactivity with DNA in the sample |
| Reaction Conditions / Protocol | Prepare gel-dye mix by adding fluorescent dye to gel matrix, filter and prime the chip on the priming station, load gel-dye mix, load RNA marker, load heat-denatured RNA samples (1 μL each, 70°C 2 min), load RNA 6000 ladder, vortex the chip, and run on the instrument; total hands-on time approximately 15 minutes with 30-minute instrument run time per chip |
| Components / Formulation | RNA 6000 Nano gel matrix, RNA dye concentrate, RNA 6000 Nano marker, RNA 6000 ladder (total RNA fragments: 0.2, 0.5, 1.0, 2.0, 4.0, and 6.0 kb), spin filters, microfluidic chips, syringe for priming station |
| Storage Conditions | Gel matrix and dye: 2–8°C protected from light (allow equilibration to room temperature 30 minutes before use); marker: 2–8°C; ladder: –70°C (heat denature before use); chips: room temperature; all components must be protected from RNase contamination |
| Shelf Life | 12 months from date of manufacture at recommended storage conditions |
| Package Specifications | 25 chips (each chip processes 11–12 RNA samples plus 1 marker and 1 ladder lane), 300 samples total per kit |
| Product Form | Liquid reagents; consumable microfluidic chips and priming equipment |
| Quality Control | Each lot tested: RIN score ≥9.5 for intact HeLa cell total RNA (RIN correlation with Northern blot confirmed); ladder resolution verified (6 peaks ≥1.0 resolution); RNA concentration linear R² ≥0.99 across 25–500 ng/μL; chip-to-chip RIN variation ≤0.5 for same RNA sample; RNase-free verified by 16-hour incubation of chip-eluted buffer with RNA substrate |
| Key Features | Objective RIN scoring eliminates subjective gel interpretation; minimal sample consumption (1 μL); automated digital data for electronic records and publication; simultaneous concentration and integrity determination; broad academic acceptance of RIN as a standardized quality metric in peer-reviewed literature |
For research use only, not for clinical use.
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