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| Product Name | Rhodamine 123 Solution, 0.5 mg/mL (CAS 62669-70-9) |
| Catalog No. | RERE-0428 |
| Description | Rhodamine 123 homes in on polarised mitochondria, accumulating in their matrix in proportion to membrane potential, and its green fluorescence makes the state of the organelle visible by flow cytometry. This ready-to-use 0.5 mg/mL stock in PBS delivers the dye at the strength most protocols expect, with excitation at 511 nm and emission at 535 nm. Light-protected refrigerated storage keeps the fluorophore stable for a year. |
| Intended Use | Flow cytometric measurement of mitochondrial membrane potential in living cells. |
| Principle / Technology | Potential-driven accumulation of the cationic dye in mitochondria scales fluorescence with membrane polarisation. |
| Specificity | 0.5 mg/mL rhodamine 123 in PBS; CAS 62669-70-9; Ex 511 nm, Em 535 nm. |
| Reaction Conditions / Protocol | Dilute into the cell suspension, incubate, wash and analyse by flow cytometry. |
| Storage Conditions | 2-8°C; protected from light; 12 months. |
| Package Specifications | 100 µL, 1 mL |
| Key Features | Pre-diluted working stock; PBS-based; two pack sizes matched to assay frequency. |
For research use only, not for clinical use.
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