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| Product Name | Rhodamine 123 |
| Catalog No. | RESK-0528 |
| Description | Rhodamine 123 (Rh 123) is a widely used cationic green fluorescent dye. It crosses the cell membrane and accumulates in the mitochondria of living cells, emitting yellow-green fluorescence, and is broadly used to detect mitochondrial membrane potential without any toxicity to cells. In addition, Rh 123 shows no fluorescence interference with common protein-labeling dyes such as PE-Cy5 and AMCA (7-amino-4-methylcoumarin-3-acetic acid), making it suitable for multiplex labeling experiments. |
| Intended Use | Detection of mitochondrial membrane potential in living cells; suitable for multiplex fluorescence labeling. For research use only. |
| Principle / Technology | As a cell-permeant cationic dye, Rhodamine 123 is taken up by living cells and accumulates in mitochondria driven by the membrane potential, producing yellow-green fluorescence (Ex/Em = 507/529 nm). |
| Detection Method | Fluorescence microscopy / fluorescence measurement (green fluorescence) |
| Sample Type | Living cells |
| Performance Range / Specifications | English synonym: 2-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic acid methyl ester, hydrochloride Formula: C21H17ClN2O3 Molecular weight: 380.82 CAS NO.: 62669-70-9 Purity: ≥98% (HPLC) Ex/Em: 507/529 nm |
| Specificity | Accumulates in mitochondria of living cells; no fluorescence interference with PE-Cy5 or AMCA. |
| Storage Conditions | Store at room temperature. |
| Shelf Life | At least one year. |
| Package Specifications | 5 mg 25 mg |
| Quality Control | Purity ≥98% by HPLC. |
| Purity | ≥98% (HPLC) |
| Molecular Weight | 380.82 |
| Shipping Conditions | Shipped at room temperature. |
| Expiration Date / Stability | Stable for at least one year at room temperature. |
| Regulatory / Compliance | For research use only. |
| Compatibility | No fluorescence interference with PE-Cy5 or AMCA (7-amino-4-methylcoumarin-3-acetic acid); suitable for multiplex labeling. |
| Application Notes / Precautions | Non-toxic to cells; suitable for live-cell mitochondrial staining and multicolor experiments. |
For research use only, not for clinical use.
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