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Rhodamine 123

Cat.No: RESK-0528 Datasheet

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Product Name Rhodamine 123
Catalog No. RESK-0528
Description Rhodamine 123 (Rh 123) is a widely used cationic green fluorescent dye. It crosses the cell membrane and accumulates in the mitochondria of living cells, emitting yellow-green fluorescence, and is broadly used to detect mitochondrial membrane potential without any toxicity to cells. In addition, Rh 123 shows no fluorescence interference with common protein-labeling dyes such as PE-Cy5 and AMCA (7-amino-4-methylcoumarin-3-acetic acid), making it suitable for multiplex labeling experiments.
Intended Use Detection of mitochondrial membrane potential in living cells; suitable for multiplex fluorescence labeling. For research use only.
Principle / Technology As a cell-permeant cationic dye, Rhodamine 123 is taken up by living cells and accumulates in mitochondria driven by the membrane potential, producing yellow-green fluorescence (Ex/Em = 507/529 nm).
Detection Method Fluorescence microscopy / fluorescence measurement (green fluorescence)
Sample Type Living cells
Performance Range / Specifications English synonym: 2-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic acid methyl ester, hydrochloride
Formula: C21H17ClN2O3
Molecular weight: 380.82
CAS NO.: 62669-70-9
Purity: ≥98% (HPLC)
Ex/Em: 507/529 nm
Specificity Accumulates in mitochondria of living cells; no fluorescence interference with PE-Cy5 or AMCA.
Storage Conditions Store at room temperature.
Shelf Life At least one year.
Package Specifications 5 mg
25 mg
Quality Control Purity ≥98% by HPLC.
Purity ≥98% (HPLC)
Molecular Weight 380.82
Shipping Conditions Shipped at room temperature.
Expiration Date / Stability Stable for at least one year at room temperature.
Regulatory / Compliance For research use only.
Compatibility No fluorescence interference with PE-Cy5 or AMCA (7-amino-4-methylcoumarin-3-acetic acid); suitable for multiplex labeling.
Application Notes / Precautions Non-toxic to cells; suitable for live-cell mitochondrial staining and multicolor experiments.

For research use only, not for clinical use.

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