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| Product Name | Reticulin Silver Stain Kit for Reticular Fibers |
| Catalog No. | ATRSA-0026 |
| Description | Silver impregnation staining kit based on the Gordon and Sweet's method for demonstration of reticular fibers (type III collagen) in tissue sections. Reticular fibers appear as fine black fibers against a pale background, with optional nuclear fast red counterstain. Essential for evaluating bone marrow architecture, liver sinusoidal framework, splenic structure, and early fibrotic changes. |
| Intended Use | Visualization of reticular fiber networks in tissue sections; bone marrow fibrosis grading (MF-0 to MF-3); liver architecture assessment; lymph node and spleen reticular pattern; early fibrosis detection before collagen becomes trichrome-positive; tumor invasion assessment. |
| Principle / Technology | Oxidation with potassium permanganate sensitizes reticular fibers; oxalic acid bleaching removes excess oxidant; iron alum sensitization; ammoniacal silver impregnation deposits metallic silver on argyrophilic reticular fibers; formalin reduction develops visible black silver precipitate; gold chloride tones; sodium thiosulfate fixes |
| Detection Method | Deparaffinize and hydrate; potassium permanganate oxidation 5 min; oxalic acid bleach 2 min; iron alum sensitization 15 min; ammoniacal silver 2-3 min (monitor microscopically); formalin reduction 1 min; gold chloride toning 1 min; sodium thiosulfate fixation 2 min; counterstain optional; dehydrate, clear, mount |
| Sample Type | FFPE tissue sections; bone marrow biopsies (decalcified acceptable); 4-5 µm sections recommended |
| Sensitivity / LOD | Detects type III collagen (reticulin) fibers at single-fiber resolution; visualizes the finest reticulin networks in bone marrow and liver |
| Specificity | Specific for argyrophilic reticular fibers (type III collagen); collagen type I (trichrome-positive) is not stained or shows minimal argyrophilia |
| Reaction Conditions / Protocol | Total protocol ~45-60 min; silver impregnation step must be monitored microscopically (2-3 min typically) |
| Components / Formulation | Potassium permanganate solution (1%), oxalic acid solution (2%), iron alum solution (2%), ammoniacal silver solution (freshly prepared components), 10% formalin solution, gold chloride solution (0.2%), sodium thiosulfate solution (5%), nuclear fast red counterstain (optional), positive control slide (normal liver) |
| Storage Conditions | 15-25 °C; silver components protected from light; ammoniacal silver prepare fresh |
| Shelf Life | 12 months for stable components; ammoniacal silver prepare fresh for each use |
| Package Specifications | Kit for 50 slides, 100 slides |
| Product Form | Liquid reagents; ammoniacal silver components mixed fresh before use |
| Key Features | Gordon and Sweet's method — gold standard for reticulin; ready-to-use stable components (except silver); includes positive control; microscopically controlled impregnation time; produces crisp black fibers on clear background; validated for bone marrow fibrosis grading per WHO criteria |
| Purity | Silver nitrate ACS grade ≥99.8%; gold chloride ≥99%; all chemicals meet histology specifications |
| Concentration | As specified; ready-to-use or concentrate format |
| Activity / Unit Definition | Staining intensity and specificity validated on reference tissue/cell preparations |
| Molecular Weight | Varies by dye component as specified in product documentation |
| Source / Origin | Synthetic dyes and biological stains; certified by Biological Stain Commission where applicable |
| pH Range / Optimal pH | pH 6.0–7.0 for Romanowsky-type stains; varies by formulation |
| Shipping Conditions | Ambient; silver nitrate is corrosive — appropriate packaging; ammonium hydroxide solution ships as corrosive liquid |
| Expiration Date / Stability | 12 months at 15-25 °C; silver nitrate solution stable when protected from light and contamination; ammoniacal silver solution must be used within 30 min of preparation; discard if ammoniacal silver develops precipitate before use |
| Regulatory / Compliance | For laboratory and research use only; RUO; silver nitrate corrosive — handle with gloves; ammoniacal silver solution may form explosive silver fulminate upon standing — always prepare fresh and discard after use; ISO 9001 |
| Compatibility | Compatible with FFPE sections from all tissue types; decalcified bone marrow biopsies stain well; avoid mercury-based fixatives; use chemically clean glassware — acid-washed and thoroughly rinsed with deionized water to prevent non-specific silver precipitation |
| Recommended Buffer System | Use with provided buffer or phosphate buffer pH 6.8 as specified |
| Application Notes / Precautions | Avoid contact with skin and clothing; stains are permanent; use appropriate PPE |
| Batch-to-Batch Consistency | Staining intensity and differential contrast validated on reference specimens per lot |
For research use only, not for clinical use.
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