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| Product Name | Red Dye for PAGE Stacking Gel (500x) |
| Catalog No. | RERE-0106 |
| Description | A ready-to-use 500x dye for preparing red coloured polyacrylamide stacking gels. The red colour makes the sample wells clearly visible for loading and easy to inspect for distortion or damage. During electrophoresis the dye compresses and migrates down through the gel to the bottom or out of it, and afterwards essentially no dye remains in the stacking gel, so it does not affect the run, Coomassie staining or transfer. Should any dye remain in the resolving gel it transfers with the proteins onto PVDF or NC membranes and then serves as a lane position marker for cutting membranes. For a 1.0 mm gel roughly 2 mL of stacking gel is needed per gel, so 1 mL of dye suffices for about 250 gels, added at 2 microlitres per millilitre of stacking gel. |
| Intended Use | Visualisation of sample wells during PAGE gel preparation and loading |
| Principle / Technology | Red dye incorporated into the stacking gel that migrates with electrophoresis without residue |
| Sample Type | Stacking gels for SDS-PAGE and native PAGE |
| Specificity | 500x concentrate added at 2 microlitres per mL of stacking gel; about 250 gels per mL |
| Reaction Conditions / Protocol | Add 2 microlitres of dye per mL of stacking gel solution before casting and polymerise as usual |
| Storage Conditions | 2 to 8°C |
| Shelf Life | 12 months |
| Package Specifications | 200 uL; 1 mL; 5 mL |
| Key Features | No interference with electrophoresis, Coomassie staining or blotting; doubles as a lane marker on membranes |
| Readouts / Downstream Assays | Visible red wells during loading and optional lane marks on the membrane |
For research use only, not for clinical use.
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