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| Product Name | Red Cell Tracking Dye (CMTPX) |
| Catalog No. | RESK-0530 |
| Description | The Red Cell Tracking Dye (CMTPX) is a cytoplasmic red fluorescent probe that readily crosses the membrane of living cells and is converted intracellularly into a cell-impermeant reaction product that emits a strong fluorescent signal. As cells proliferate through passages, the dye is transferred to daughter cells rather than to adjacent cells in the population, and the fluorescent signal is maintained for at least 72 h, demonstrating excellent tracing performance. The dye is non-toxic and stable, provides a long-lasting fluorescent signal, and exhibits bright fluorescence under physiological pH conditions. With excitation and emission maxima of Ex = 577 nm and Em = 602 nm, it produces red fluorescence that pairs well with green fluorescent protein (GFP), enabling multiplexed fluorescence detection. |
| Intended Use | Fluorescent tracing of living cells for monitoring cell proliferation and tracking cells across generations. |
| Principle / Technology | The dye freely permeates the membrane of living cells and is converted inside the cell into a membrane-impermeant reaction product with strong red fluorescence. Upon cell division the dye is passed to daughter cells instead of neighboring cells, and the signal persists for at least 72 h, supporting multi-generation cell tracking. |
| Detection Method | Fluorescence detection with Ex = 577 nm and Em = 602 nm (red fluorescence); suitable for multiplexed fluorescence analysis together with GFP. |
| Sample Type | Living cells. |
| Performance Range / Specifications | Fluorescent signal is maintained for at least 72 h and is inherited by daughter cells during proliferation. |
| Specificity | Labels the cytoplasm of living cells; dye is transferred to daughter cells rather than adjacent cells in the population. |
| Reaction Conditions / Protocol | Dissolve the powder in DMSO to prepare a stock solution, then dilute into buffer (e.g., PBS) to prepare the working solution. Detectable fluorescence of the working solution in PBS is normal, as the product itself is fluorescent; after entering living cells it is converted to a membrane-impermeant product emitting strong, bright red fluorescence. |
| Components / Formulation | Single-component fluorescent dye supplied as a purple solid powder; readily soluble in DMSO. |
| Storage Conditions | Powder: store dry at -25 to -15 °C. After dissolution in DMSO, aliquot the stock solution and store at -25 to -15 °C protected from light; avoid repeated freezing. |
| Shelf Life | Powder: 18 months when stored dry at -25 to -15 °C. DMSO stock solution (aliquoted, protected from light, -25 to -15 °C): 6 months. |
| Package Specifications | 50 μg 10 x 50 μg |
| Product Form | Purple solid powder. |
| Purity | >89% |
| Molecular Weight | 686.25 |
| Source / Origin | Chemical synthesis. Molecular formula C42H40N3O4Cl. |
| pH Range / Optimal pH | Bright fluorescence under physiological pH conditions. |
| Shipping Conditions | Shipped at room temperature. |
| Expiration Date / Stability | Powder is stable for 18 months when stored dry at -25 to -15 °C; DMSO stock solution is stable for 6 months when aliquoted and stored at -25 to -15 °C protected from light. Intracellular fluorescent signal persists for at least 72 h. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Can be used together with green fluorescent protein (GFP) for multiplexed fluorescence detection. |
| Recommended Buffer System | Prepare a stock solution in DMSO; dilute into PBS or a suitable buffer to prepare the working solution. |
| Application Notes / Precautions | The dye is non-toxic and stable with a long-lasting signal, and shows bright fluorescence at physiological pH. Aliquot the DMSO stock solution and avoid repeated freezing. Store protected from light. |
For research use only, not for clinical use.
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